六味地黄丸对自发性糖尿病大鼠胰腺的保护作用.PDFVIP

2010;30(6) 南方医科大学学报(JSouthMedUniv) 窑1407窑 六味地黄丸对自发性糖尿病大鼠胰腺的保护作用 李 佳袁薛耀明袁钱 毅袁朱 波袁潘永华袁张 燕渊南方医科大学南方医院内分泌代谢科袁广东 广州 510515冤 摘要院目的通过观察六味地黄丸对自发性2 型糖尿病OLETF大鼠胰腺形态学的影响袁探讨六味地黄丸预防糖尿病的 可能机制遥 方法 自发性2 型糖尿病大鼠OLETF 鼠40 只袁随机分为六味地黄丸干预组和对照组袁LETO 鼠10只作为正 常对照组遥干预组从 -1 -1 8周龄起以六味地黄丸2.4mg窑kg 窑d 灌胃给药袁其余两组以等量清水灌胃遥定期OGTT试验遥于 8尧32 和40 周龄时分批宰杀大鼠遥分离胰腺遥HE染色观察胰岛结构曰Masson 三色染色观察胰岛纤维化程度遥结果 随 着周龄的延长袁对照组大鼠胰岛逐渐出现纤维化袁萎缩曰干预组的胰岛结构保存明显好于对照组遥 通过Masson染色袁可 见干预组胰岛纤维化程度明显轻于同周龄对照组遥LETO组大鼠胰腺结构随着周龄延长无明显变化遥干预组从30周龄 开始出现糖尿病袁 至40周糖尿病发病率28.6%袁 显著低于对照组 (P0.01)遥 结论 六味地黄丸早期干预能明显保护 OLETF大鼠胰岛结构减少其纤维化袁可能是其预防糖尿病的机制之一遥 关键词院2 型糖尿病曰六味地黄丸曰OLETF 鼠曰胰腺曰组织学 中图分类号院R587 文献标识码院A 文章编号院1673-4254(2010)06-1407-03 EffectsofLiuweidihuang pillsonpancreaticisletstructureinOLETFrats LIJia,XUEYao-ming,QIANYi,ZHUBo,PANYong-hua,ZHANGYan DepartmentofEndocrinologyandMetabolism,NanfangHospital,SouthernMedicalUniversity,Guangzhou510515,China Abstract:Objective TostudytheeffectsofLiuweidihuangpillsonpancreaticisletstructureinOLETF(OtsukaLong-Evans TokushimaFatty)rats.MethodsFortymaleOLETFratsweredividedrandomlyintoLiuweidihuangpillsgroup(Liuweigroup) andcontrolgroup(n=20). TenmaleLETOratswereusedasnormalcontrolgroup(LETOgroup).TheratsinLiuweigroup weregiven Liuweidihuang pillsatthedailydoseof2.4mg/kgintragastricallysincetheageof8weeks. Bloodglucosewas determinedbyOGTT. Theratsweresacrificedat8, 32and40weeksandthepancreatictissuewasisolatedtoexaminethe morphological changes of the pancreas by HE staining and Masson trichrome staining. Results As the age of the rats increased, thepancreaticisletsinthecontrolgroupgraduallyshowedfibrosisandisletatrophy, whichwerenotfoundin Liuweigroup. MassonstainingvisualizednofibrosisinLiuweigroup. Nosignificantpathologicalchangeoccurredinthe pancreaticisletofLETOrats. TheratsinLiuweigroupdevelopeddiab