细菌脱氢酶活性生物测试法用于水质毒性检测的改进.pdfVIP

中国环绕舷测 Vol. 26 No.3 第 26 卷第3 期 2010 年6 月 Environmental Monitoring in China Jun. 2010 细菌脱氯酶活性生物测试法用于水质毒性检测的改进 陈 莉，粱文袍，李 炯，郭思远(北京林业大学环境科学与工程学院，北京 10∞83) ， 摘 .:从培养基配方、简种来源、激活时间、筒液用J:以及检测方式等几个方面对蜕氢酶快速毒性检测法进行了改进， 确定1.个体系的操作方法。并用 HgCl NaN 段低了改进后方法的可行性。结果农明，以天然地农水作为篱种来 和 2 1 1 次后，混合细菌的脱氯酶治性的稳定性便可达到实验要求。样，g，液与前液按照 6:1 的比例混合效果较 源，转接螃养 好。混合体系在 37C但滋生寄养箱中激活 50min 以上细菌可完合治化，最终检测可用普通分光光皮计进行。改进后的方 法解决了，i方法西自浪尖活过快，实验稳定性较差的问题，同时克服了枪测仪部难以购买的问题，使这一方法得以在普通 实验室实现。 关键词:脱氮路;混合细菌:惑，院检测;生物测试 中回分类号: X832 文献标识码:A 文章编号:1∞2-6002 (2010 )03心017-05 Improvement on the Determining Metbod of Debydroge翩翩se Activity In Water To对clty CH配N Li, et al (College of Environmental Science Engineering , Beijing Forestry University , Beijing 100083 , China) Ab由tract: The paper studied the bio8ssay method of dehydrogenase activity and ímproved the experiment condítions. 8uch ωthe cultrure medíum , the source and amount of bacteria , the 8ctivation tíme 8S well as detection method. The operating conditions and method were determined. The feasibility of the improved method was veri臼ed by the bioassay of HgCl2 and NaN 3 • The results showed that when natural surface water was used a8 bacteria source , the dehydrogenase activity of bacteria could keep higher after transferring culture for one time. The optimum mixing ratio of water sample ωbacteria suspension liquíd was 6: 1 (V: V ) . Bacteria activíty could be fully activated when mixed system of preserved bacteria and cultrue medium were placed in 37 C for more than 50min. The improved method overcame the short pre脚rvatin time and stability for bacteria and could be realized in ordinary laboratory by the use of spectrophotometer. Key words: Dehyd