苏南地区1b型丙型肝炎病毒包膜区变异进化研究.pdfVIP

第 21 卷 4 期 中 国 病 毒 学 21 （4 ）：333-338 2006 年 7 月 VIROLOGICA SINICA July 2006 苏南地区1b 型丙型肝炎病毒包膜区变异进化研究* 1 1 2 3 3 1,2** 许 可 , 蒋春梅 , 邓小昭 , 丁伟良 , 谈永飞 , 张 云 （1.南京医科大学流行病与卫生统计学系，江苏南京 210029 ；2.南京军区军事医学研究所，江苏南京 210002 ；3.宜兴市第一人民医院， 江苏无锡 214200 ） Study On the Variation of E2 of 1b Hepatitis C Virus in the South of Jiangsu Province 1 1 2 3 3 1,2** XU Ke ，JIANG Chun-mei ，DENG Xiao-zhao ，DING Wei-liang ，TAN Yong-fei ，ZHANG Yun (1. Department of Epidemiology in Nanjing Medical University, Nanjing 210029, China; 2.The institute of Militalry Medicine, Nanjing Command, Nanjing 210002 ，China; 3. Yixing people’s hospital,Wuxi 214200, China.) Absract ：The aim of this study was to investigate the variation of the second envelope glycoprotein E2 of Hepatitis C Virus strains from the south of Jiangsu province and to learn about the relationship between HCV quasispecies and alanine aminotransferase (ALT). Identification genotypes of sera samples obtained from 166 donors who were HCV positive was carried our by the PCR with type specific primers based on the sequence of 5′non-coding region (5 ′NCR). Forty-three chronic 1b type hepatitis C patients who had not received any antiviral therapy were categorized into two groups according to the ALT level. HCV quasispecies diversity were determined by RT-PCR single-st