糖链的生物质谱分析 - 生命科学.pdf

第23卷第6期 生命科学 Vol. 23, No. 6 2011年6月 Chinese Bulletin of Life Sciences Jun., 2011 文章编号：1004-0374(2011)06-0569-08 糖链的生物质谱分析 王承健，王仲孚* (西北大学生命科学学院，西部资源生物与现代生物技术教育部重点实验室，西安710069) 摘　要：糖链是重要的生物信息分子，在许多生理和病理过程中都发挥着独特作用。糖链结构非常复杂， 具有微观不均一性，其分析和结构解析一直是糖生物学研究的瓶颈。质谱具有灵敏度高、可获得多种结构 信息和适于分析混合物等优点，是糖链定性定量分析的一种理想手段。电喷雾电离质谱和基质辅助激光解 析电离质谱两大生物质谱技术已被广泛应用于糖链的相对分子质量指纹谱分析、序列和连接方式测定及相 对定量分析。对近年来以质谱为主要分析手段的糖链分析方法研究进展做一综述。 关键词：糖链 ；结构；质谱 中图分类号：Q53; O657.63 文献标志码 ：A Glycan analysis by biological mass spectrometry WANG Cheng-Jian, WANG Zhong-Fu* (Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Sciences College, Northwest University, Xi’an 710069, China) Abstract: As a group of bioinformation molecules, glycans play significant roles in many physiological and pathological processes. Due to their structural complexity and microheterogeneity, analysis and structural characterization of glycans are the bottleneck of glycobiology research. Mass spectrometry, which has advantages in the ability to minimize sample consumption, obtain diverse structural information, and analyse mixtures, is an ideal device for qualitation and quantitation of glycans. The two biological mass spectrometry technologies, electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), have been widely used in the analysis of glycan molecular mass fingerprint, sequence, linkage, and relative quantitation. This article summarizes the recent progress in the methodology investigation of glycan analysis using mass spectrometry as a