锂-匹罗卡品癫模型中核转录因子的表达及干预机制.pdfVIP

· 326 · Stroke and Nervous Diseases，Dec 2007 Vo1．14，No．6 ， 锂一匹罗卡品癫痫模型中核转录因子的表达及 干预机制 杨银升 杨金升 石向群 丁小涵 陈合成 【摘要】 目的 探讨癫痫发作时核转录因子(nuclear factor kappaB，NF-~B)在神经元损伤中的表达变化 以及拉莫三嗪、黄芪注射液对这一过程的影响。方法 干预组大鼠预处理3 d后制作大鼠锂一匹罗卡品(1ithi— um-pilocarpine，Li—PC)癫痫模型，造模后24 h、3、7 d应用光镜及电镜进行神经元形态学观察，利用免疫组织 化学法半定量检测NF-~B的表达含量。结果 与正常组比较，模型组海马内NF--cB表达明显增多(P 0．【)1)，在24 h达峰值；与模型组比较，拉莫三嗪、黄芪注射液能够使NF-~B表达增加(P()．05)。结论 NF- -cB的主要作用是有利于神经元生存，拉莫三嗪、黄芪注射液可通过调控凋亡因子的表达而发挥对癫痫的神经 元保护作用。 【关键词】 锂一匹罗卡品癫痫模型 NF一．cB 拉莫三嗪 黄芪 【中图分类号】 R742．1 【文献标识码】 A 【文章编号】 1007—0478(2007)06—0326—04 Expression nuclear factor kappa B in the rat lithium-piIocarine model Yang Yinsheng，Yang J insheng，Shi Xiangqun，el a1．Department of Neurology，The Lanzhou General Hospital of PLA，Lanzhou 730050 [Abstract] Objective To explore the role of nuclear factor kappaB(NF-~cB)during neuronal damage， and the contribution of lamotrigine，Huangqi in epileptic pathogenesis．Methods After the rats preconditioned for 3 days in the intervention group and the rat lithium-pilocarpine(Li—PC)model was established except the control group，surveying the morphology of neuron by light and electron microscope，detecting the contents of Nuclear Factor kappaB by semiquantitative irnmunohistochemical method after SE 24 h，3 d and 7 d．Results Compared with the control group in hippocampus，expressions of NF-~B increased(P()．01)peaked at 24 l1． With the model group，the NF-~B activation values positively correlated in all intervention groups(P()．05)． Conclusions NF-~B is contributed to the survival of neuro~Lamotrigine and Huangqi could protect neuron by regulating apoptosis factors． [Key wor~] Lithium-pilo