仙鹤草降糖有效部位筛选研究.pdfVIP

现代食品与药品杂志 2007年第l7卷第6期 · 15· · 中药与天然药物· 仙鹤草降糖有效部位筛选研究 万 波 ，昊 莹 ，曾英彤 (1．广东省人民医院药学部，广东广州510080；2 华中科技大学同济医学院药学院，湖北武汉430030) 摘 要：目的 筛选仙鹤草降糖作用的有效部位，并初步探讨其降糖机制。方法 分别选正常小鼠、四氧嘧啶糖尿病小鼠模 型和由葡萄糖灌胃高血糖小鼠模型，观察比较仙鹤草的水、95％乙醇、石油醚和乙酸乙酯提取部位的降糖效果，灌胃剂量为 2．0 g·kg一·d一，采用苯乙双胍为阳性对照，葡萄糖氧化酶法测定血糖。结果 仙鹤草乙醇提取部位连续给药7 d，可显著 降低正常小鼠血糖值，与正常对照组比较，血糖降低水平有显著性差异(P0．05)；仙鹤草乙醇提取物能显著降低四氧嘧啶糖 尿病小鼠的血糖，与模型组比较，有极显著性差异(P0．01)；仙鹤草乙醇提取物能显著降低葡萄糖灌胃高血糖小鼠模型血 糖，与模型组比较，有显著性差异(P0．05)。结论 仙鹤草乙醇提取部位为有效降糖部位。 关键词：仙鹤草；血糖；有效部位 中国分类号：R284 文献标识码：A 文章编号：1673—4610(2007)06—0015—03 A Screening Study of Hypoglycemic Effect of Herba Agrimoniae WAX Bo ，wu Ying2，ZENG Yin-tong (I．Guangdong Provincial Peopleg Hospital，Guangzhou，Guangdong 5 1 0080；2．Ton6i Medical Cortege of Huazhong University of Science and Technology，Wuhan，Hubei 430030) Abstract：0bjective To screen the effective fraction of Herba Agrimoniae with die hypoglycemic activity，and discuss its hypoglycemic mechanism．Methods Use water，95％ alcohol，ethyl acetate and petroleum ether to extract the Herba Agrimoniae Normal，alloxan- induced diabetic mellitus．glucose-induced hyperglycenfic mice were given 2 0 g·kg～·d一 of the extract of Herba ngrimoniae and the positive control groups were given the sallle volume of Phenformin．Blood glucose levels of the animals in the course of the experiment were monitored．Results The alcohol extract of Herba ngrimoniae was shown to lower the blood glucose level of the normal mice after 7 days of treatment．Compared with the control group，the difference was significant(P0．05)．The alcohol extract of Herba Agrimoniae could strikingly reduce the blood glucose of the alloxan-induced diabetic mice，after 7 days of treatment．Compared with the model group，the difference was significant(P0，01)．The alcohol extract of Herba A moniae could reduce the blood glucose of the glucose-induced hyperglycemic mice，compared with the model group，the difference was significant(P0．05)．