氢化物发生-原子荧光光谱法测定鲜黄蘑菇中的硒.pdfVIP

ofAnhui 安徽农业科学，Journal Agi．Sei．2014，42(19)：6387，6480 责任编辑李菲菲责任校对李岩 氢化物发生一原子荧光光谱法测定鲜黄蘑菇中的硒 胡兰基1，杨娜1，王洪桂1，霍成玉1，-6文2(1．青海省地质矿产测试应用中心，青海西宁810008；2．青海省地质调查局，青海 西宁810001) 摘要[目的]快速、准确地测定鲜黄蘑菇中的硒含量。[方法]采用新鲜黄蘑菇直接打成浆液后经硝酸和高氯酸微波消解，用氢化物发 生一原子荧光光谱法测定消解液中硒的含量。[结果]鲜黄蘑菇样品经硝酸和高氯酸微波消解后，所得消解液在6mol／L盐酸介质中煮 沸3～5 rain使硒(VI)完全还原成硒(IV)，用Fe“盐作为抑制剂，氢化物发生器在还原剂为20∥L的硼氢化钾(5g／LKOH介质)，栽流 为5％～10％HCl时进行测定。氢化物发生一原子荧光光谱法测定鲜黄蘑菇中的微量硒，硒的质量浓度在20一L以内与荧光强度呈 线性关系，方法检出限(3 定鲜黄蘑菇中的硒含量。 关键词氢化物发生一原子荧光光谱法；鲜黄蘑菇：硒 中图分类号$646文献标识码A 文章编号0517—661l(2014)19—06387—01 HG-AFSDeterminationofSeleniuminFreshYellowMushroom HU et ResearchInstituteof and Lan-jial(Qinghm GeologicalMinerM，Xining，Qinghai810008) Abstract To and determineseleniumcontentinfresh offresh mush— 『0biectiverapidlyaccurately yelowmushroom．『MethodSample yellow roomwas with was to in offresh HNO，andHCl0。，HG—AFSmethoduseddetermineseleniumsolution．fResult mush— digested Sample yellow roomwas with and solution for3—5minutesafteradditionof6mol／LHCltoreduceSe HN01HCl04．111e digested sample keptboilinggently to takenfortheHG—AFSdeterminationofFe”for was Se(IV)．Thesolutionwas addition (Ⅵ)completely s砌ole inhibitor．Hydridegeneration in for20 of KOH for HCl．HG—AIrSdeterminationof 5％一10％of performedreducingagent g／L KBH4(5g／L medium)andcurrent—carrying traceseleniuminfresh mushroomthestandardcurvemethod．Theoftheseleniumconcentrationwithin20 hasalinear yellow using quality ug／L relationinfluorescence Values 2．18％for