益智胶囊中9种有效成分的UPLC-MSMS法测定.pdfVIP

·381· JournalofPharmaceuticals 中国医药工业杂志Chinese 2015，46(4) 益智胶囊中9种有效成分的UPLC．MS／MS法测定 李雪娜1，欧阳明“，刘晓燕2…，李向军1滞，刘敏彦2 (1．黑龙江中医药大学，黑龙江哈尔滨150000；2．河北以岭医药研究院有限公司，河北石家庄050035； 摘要：建立了超高效液相色谱串联质谱法同时检测益智胶囊中的9种成分一一原儿茶醛、绿原酸、紫丁香苷、隐绿原酸、 甲酸为流动相，梯度洗脱，负离子模式检测。结果9种有效成分在检测的范围内线性关系良好，方法回收率为97．7％～ 101．1％，RSD均小于3％。 关键词：益智胶囊；超高效液相色谱串联质谱；测定 中图分类号：R917；0657文献标志码：A 文章编号：1001．8255(2015)04—0381．04 inYizhi ofNineActiveConstituents QuantitativeAnalysis Capsuleby LI Xuenal，OUYANGYue2”，LIU Xiaoyan2”，LIXiangjunl，抖，LIUMinyan2 Chinese Pharmaceutical Medicine,Haerbin150000,2．Institute Co．，Ltd．， (J．HeilongfiangUniversityofTraditional ofHebeiYiling Shijiazhuang Disease 050035) 4．KeyLab．ofNetworkofHebeiProvince,Shijiazhuang methodwasestablishedforthesimultaneousdeterminationofnineactive ABSTRACT：AnUPLC—MS／MS inYizhi aldehyde，chlorogenic acid， compoundscapsule，includingprotocatechuic acidBandsalvianolicacidA。AWaters UPLCBEH echinacoside，verbascoside，rosmarinicacid，salvianolic Acquity CIR columnwasusedwiththemobileof formicacid elution．The of phaseacetonitrile：0．1％aqueousbygradient monitoring all wasachievedunderthe ionizationmode．A11calibrationcurvesshowed withinthetest analytes negative goodlinearity in the recoverieswerethe of97．7％．101．1％，wim脚Slessthan3％． r