微波法提取油茶叶总黄酮的工艺研究.pdfVIP

安徽农业科学．Journal ofAnhui Agri．Sci．2007，35(35)：11372—11374 责任编辑 张杨林 责任校对 王森 微波法提取油茶叶总黄酮的工艺研究 龚建良 ，李姣娟2，陈丛瑾 (1、中南林业科技大学材料科学与工程学院，湖南长沙 410004；2．中南林业科技大学应用化学研究所，湖南长沙 410004) 摘要 『目的1为油茶资源的高效开发和综合利用提供一些参考依据。f方法1利用微波法提取油茶叶中的总黄酮，探讨了乙醇浓度、固 液比、提取温度、提取时间和提取次数等因素对总黄酮得率的影响，通过单因素试验和正交试验确定了提取的最佳工艺条件。f结果1 在单因素试验中，油茶叶总黄酮得率随微波作用时间的增加先升后降，随着乙醇浓度的增加先增大后减小，随温度的升高而升高，但 过高温度会使得率降低。正交试验、方差分析及显著性检验结果表明：4个因素对总黄酮得率的影响大小依次为：乙醇浓度提取温 度固液比提取时间。微波法提取油茶叶总黄酮的最佳工艺条件为：用5O％乙醇，固液比l：20，在8O℃条件下提取8min，连续提取 2次，总黄酮得率为48．53 mg／g。『结论1微波提取法能有效降低生产成本，提高经济效益。 关键词 油茶叶；总黄酮；微波提取；最佳提取工艺 中图分类号 $571．1 文献标识码 A 文章编号 O5l7—66l1(2007)35一l 1372—03 Research on the Technology of Extracting Total Flavonoids from Camellia D ifera Leaves by Microwave Method GONG Jian-liang et al(College of Materials Science and Engineering，Central South University of Forestry and Technology，Changsha，Hunan 410004) Abstract 『0bjective1 The aim of the study was to provide some referenee foundation for the high-efficient development and comprehensive utilization of Camellia ole ra resources、fMethod1 Total flavonoids were extracted from C oleifera leaves by microwave method．The influences of the factors such as ethanol concentration，ratio of solid to liquid，extraction temperature，time and frequency on the yield of total flavonoids were discussed．The optimum technic condition for the extraction was confirmed through single-factor experiment and orthogonal experiment．【Result】 In the single-factor experiments，the yield of total flavonoids from C ole ra leaves increased firstly and then decreased along with the increment of time of microwave and ethanol concentration．increased along with the increment of temperature．but too high temperature would decrease the yield．The results from orthogonal experiment．variance analysis and significance test showed that the influence order of 4 factors on the yield of total flavonoids was ethanol concentrationextracti