固定化优势菌PM1降解甲基叔丁基醚.pdfVIP

中国环境科学 2007，27(6)：78 1~785 China Environmental Science 固定化优势菌PM1降解甲基叔丁基醚 成卓韦，陈东之，章晶晓，钟卫鸿，陈建孟’(浙江工业大学生物与环境工程学院，浙江杭州310032) 摘要：采用海藻酸钙固定化优势菌Methyl~mm petroleipmhilu PM1降解甲基叔丁基醚(MTBE)．结果表明，固定化细胞对于pH值、温度、 底物浓度的适应范围变宽，在pH5～lO、温度25--40C、底物浓度10-500mg／L时，固定化细胞具有较高的MTBE生物降解活性；30~C储存降 解活性半衰期为96h；重复使用3O批活性没有明显降低；扫描电镜观察表明，细胞在海藻酸钙包埋载体中能良好地生长和繁殖．固定化细胞降 解^，rrBE反应符合零级动力学特征． 关键词：甲基叔丁基醚：Methylibiumpetroleiphilum PM1；固定化细胞；海藻酸钙；废水处理 中图分类号：X703 文献标识码：A 文章编号：1000—6923f2007)06—0781—05 Biodegradation of methyI tert-butyl ether by immobilized predominant bacteria PMI．CHENG Zhuo·wei．CHEN Dong-zhi，ZHANG Jing-xiao，ZHONG Wei-hong，CHEN Jian-meng(College ofBiological and Environmental Engmeefing， Zhejiang UniversityofTechnology,Hangzhou 310032，China)．ChinaEnvironmentalScience，2007，27(6)：781-785 Abstract：Methyl tert-butyl ether(MTBE1 was biodegradated by calcium alginate immobilized Methylibium petroleiphilum PM 1．The adaptable range of immobilized cells on pH value，temperature and initial MTBE concentration Was widened．When pH value Was 5 to 10，temperature Was 25 to 40℃，initial concentration Was 10 to 500mg／L，the immobilized cells had higher MTBE-bidegradation activity．The halGlife of MTBE·degradation activity for immobilized cells stored at 3O℃ Was 96 h，and its activity did not decrease significantly after repeated use for 30 cycles．Scanning electron microscope demonstrated that immobilized cells could grow and breed well in calcium alginate carrier．MTBE degradation by immobilized cells followed zero order kinetic characteristic． Key words：methyl tert-butyl ether；Methylibium petroleiphilum PM1；immobilized cells；calcium alginate；wastewater treatn1cnt 甲基叔丁基醚 (MTBE)是一种汽油添加剂，通过包埋PM1降解MTBE，研究该菌株在固定化 可以减少汽车尾气中 CO的排放【J J．由于 MTBE 条件下的降解性能，并对降解过程的动力学进行 具有较高的水溶性和较低的亨利系数，可与苯、 分析． 甲苯、乙苯等产生共溶，对水体产生污染，美国 1 材料与方法 EPA已将其列为潜在的人类致癌物 J．近年来，国 外对 MTBE污染治理技术的研究极为重视，其 1．1 材料 中，微生物降解法