第13讲蛋白质组分析汇编.ppt

* Isoelectric points and molecular weights are characteristics completely independent from each other; Each protein has a specific pI and MW You can detect up to 5,000 protein spots in one gel. * The traditional technique has been introduced in 1975 by the two authors independently:O’Farrell PH. J Biol Chem. 250 (1975) 4007-4021. Klose J. Humangenetik. 26 (1975) 231-243. The main steps are: Extraction or dilution of proteins with lysis buffer containingSDS and 2-mercaptoethanol. Sample loading at the cathodal side. All proteins should go into solution, prevention of protein aggregates and