姜黄素对2型糖尿病大鼠肾脏改变的影响.pdfVIP

医药导报2007年7月第26卷第7期 ·749· 姜黄素对2型糖尿病大鼠肾脏改变的影响 3,1慎微 ，熊礼佳 ，付艳红 (华中科技大学同济医学院1．附属同济医院‘肾内科；2．2004级硕士班，武汉 430030) [摘 要] 目的 探讨姜黄素对大鼠糖尿病肾病的疗效及其作用机制。方法 将大鼠随机分为假手术组(剥离左 侧肾包膜)和单侧肾脏切除组、糖尿病模型组及姜黄素治疗组各8只(均切除左侧肾脏)。于手术后2周开始，均给予高 脂高糖饮食诱导4周。糖尿病模型组与姜黄素治疗组分2次小剂量腹腔注射链脲佐菌素，30 mg·kg一；假手术组和单侧 肾脏切除组腹腔注射等量枸橼酸缓冲液。均间隔l周。第2次注射后2周，测定各组大鼠血糖(Bs)、肌酐清除率(Ccr)、 尿微量清蛋白(mAlb)、肾组织中核因子一KB p65及Toll样受体2(TLR2mRNA)表达水平，观察肾脏形态变化。结果 姜 黄素治疗组Ccr、mAlb较糖尿病模型组明显下降，Bs无明显差异。姜黄素治疗组NFKB p65在核内表达水平较糖尿病组 明显下降，而TLR2mRNA表达无明显差异。光镜下姜黄素治疗组肾组织的病理变化较糖尿病模型组明显改善。结论 姜黄素可明显改善糖尿病大鼠的肾脏病理变化，其作用机制可能与抑制NF-zB p65的核转导有关，但对TLR2的表达没 有抑制作用。 [关键词] 姜黄素；糖尿病肾病；核因子一zB；Toll样受体 [中图分类号] R286；R965．2 [文献标识码] A [文章编号] 1004-0781(2007)07-0749-05 Effect of Curcumin on Kidneys in Type 2 Diabetic Rats uu Shen—wei，XIONG Li-jia，Fu Yan—hong(Department ofNephrology，Tongji Hospital Affiliated with Tongji Medical College，Huazhong University ofScience and Technology，Wuhan 430030，China) ABSTRACT 0bjective To investigate the effect and mechanism of curcumin on renal lesions in experimental type 2 diabetic rats． Methods Diabetic rats were randomly divided into sham operated group，uninephrectomized group，diabetic group and diabetic group treated with curcumin with 8 rats in each group．Two weeks after operation rats were given the diet rich in fat and sugar for 4 weeks．The rats were injected twice with one week interval intraperitoneally with low dose of streptozotoein 3O mg·kg～ in diabetic group and curcumin—treated group，or citrate buffer in sham—operated group and uninephrectomized group．Two weeks after the second injection the blood sugar，creatinine clearance，24 h urinary albumnin excretion，the expression of NFKB(immunohistochemistry)and TLR2mRNA(RT—PCR)as well as renal morphology were determined