中药饮片中二氧化硫残留量的快速检测方法.pdf

中药饮片中二氧化硫残留量的快速检测方法  吴华庆 1 ，杨华剑 2 ，吴晓平 2*(1.浙江英特集团股份有限公司，杭州 310015 ；2.浙江省临海市食品药品检验检测中心，浙江 临海 317000) 摘要：目的 探索中药材中二氧化硫残留量的快速检测方法。方法 考查碱性超声波浸提法和蒸馏法对二氧化硫提取效 果的影响。无色或白色样品，采用碱浸提 30 min ；有色样品采用蒸馏法提取。并采用检测试剂盒法测定。结果 二氧化 硫线性范围为 1~12.0 g(r=0.999) ，检出限为 0.21 g 。其中碱性超声波浸提法的重复性 RSD 为 1.0%，样品加样回收率为 91.2%~95.4% ；蒸馏法的重复性 RSD 为 1.3%，样品加样回收率 90.3%~98.2%。36 份不同样品测定结果与中国药典 2015 年版方法测定结果基本一致。结论 该快速检测方法具有简便、快速、准确、经济等特点，适合在中药材收购部门、中 药饮片生产经营企业、监管部门推广应用。 关键词：中药饮片；二氧化硫；快速检测；检测试剂盒 中图分类号：R917.105 文献标志码：B 文章编号：1007-7693(2016)06-0775-03 DOI: 10.13748/ki.issn1007-7693.2016.06.023 Rapid Detection Method of Sulfur Dioxide Residue in Chinese Herbal Medicines 1 2 2* WU Huaqing , YANG Huajian , WU Xiaoping (1.Zhejiang Intec Group Company Limited, Hangzhou 310015, China; 2.Linhai Center for Food and Drug Control, Linhai 317000, China) ABSTRACT: OBJECTIVE To establish a rapid detection method of sulfur dioxide residue in Chinese medicinal materials. METHODS Sulfur dioxide was extracted by two different methods from the samples and then detected by the test kit. For the colorless sample, sulfur dioxide was extracted with aqueous alkyl after 30 min sonication. For the dark color sample, sulfur dioxide was extracted with distillation. RESULTS The linearity of SO2 ranged 112 g(r=0.999). The limit of detection was 0.21 g. For the sonication method, the RSD was 1.0%. Recoveries of spiked samples varied from 91.2% to 95.4%. For the distillation method, the RSD was 1.3%. Recoveries of spiked samples varied from 90.3% to 98.2%. Thirty-six different herbal samples were detected. The present method produced similar results compared to the Chinese Pharmacopoeia analysis. CONCLUSION The rapid detection method is simple, rapid,