耳蜗核前腹核丛细胞的功能修饰先于听觉的建立-南方医科大学学报.PDFVIP

J South Med Univ, 2014, 34(7): 923-927 doi 10.3969/j.issn. 1673-4254.2014.07.02 ·923 · 基础研究 耳蜗核前腹核丛耳蜗核前腹核丛细胞的功能修饰先于听觉的建立细胞的功能修饰先于听觉的建立 钟圣辉，张正刚，傅鸣宇，杨 娟，肖中举 南方医科大学基础医学院生理学教研室，广东 广州 510515 摘要：目的通过全细胞膜片钳技术研究新生SD乳鼠耳蜗核前腹核（AVCN）中丛细胞在发育过程中的电生理特性变化。方法 运用全细胞膜片钳记录出生后5~21 d 的SD 乳鼠耳蜗核前腹核中丛细胞的动作电位（AP）反应及微小兴奋性突触后电流 （mEPSC），再通过相关数据采集及分析软件分析动作电位半峰宽和mEPSC 的10%~90%上升时间（10%~90% Risetime）及时间 常数，并对此做进一步统计分析处理，进而比较不同年龄段新生SD乳鼠的耳蜗核前腹核中丛细胞的电生理反应特性。结果 在 丛细胞动作电位半峰宽的统计分析中发现，丛细胞动作电位的时间精确性随着出生后年龄的增加而增加，在听觉建立（出生后 12 d）附近变化停止。丛细胞mEPSC 的时间精确性也随着出生后年龄的增加而增加，在听觉建立附近变化停止。结论 耳蜗核 前腹核中丛细胞功能的修饰先于听觉的建立。 关键词：耳蜗核前腹侧核；丛细胞；发育；电生理 Functional refinement of bushy cells in the anterior ventral cochlear nucleus precedes hearing onset in neonatal rats ZHONG Shenghui, ZHANG Zhenggang, FU Mingyu, YANG Juan, XIAO Zhongju Department of Physiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China Abstract: Objective To investigate the development of the electrophysiological property of bushy cells in the anterior ventral cochlear nucleus (AVCN) of neonatal Sprague Dawley (SD) rats. Methods The development of action potential and spontaneous miniature excitatory postsynaptic currents (mEPSCs) in AVCN bushy cells were investigated by whole-cell patch-clamp technique in SD rats during the postnatal days 5-21 (P5-21). The half band width of the action potential (AP), 10%-90% risetime and decay tau of the mEPSCs were analyzed. Results The AP of the bushy cells became faster with age from P5 to P21 and stopped changing around the period of hearing onset, as evidenced by the alteration of half band width of the AP. The time accuracy of mEPSCs of the bushy cells also increased with age and stabilized around hearing onset as shown by briefer 10%-90% rise time and decay tau of mEPSCs in P14/P21 than in P7. Conclusion The functional refinement of the bushy ce