超声微泡介导bdnf基因联合转染大鼠视网膜和视 - 第三军医大学学报.docVIP

Ultrasound-mediated BDNF gene transfection to the rat retina and visual cortex enhanced the RGCs protection effect after optic Nerve Injury Fu Yi，Liu Su，Wang Zhigang et al. Department of Ophthalmology，2nd Affiliated Hospital,Chongqing Medical University, Chongqing 400010, China Corresponding author: Liu Su, Email: liusu2836@ Abstract Objective: Transfected BDNF gene to the rat retina and visual cortex by ultrasound-mediated and estimate the protection effect on the RGCs. Methods: SD male rats 88, were randomly divided into six groups, namely A normal groups (8), B sham operation groups (16), C control groups (16), D eyes transfection groups ( 16), E brain transfection groups (16), F co-transfection groups (16). After 1 week and 2 weeks of the injury, use Western Blot to test the BDNF expression, retrograde fluorogold labeled RGCs to counte the cell number, PERG to text the visual function of RGCs. Results: BDNF succeeds expressing after tranfection; 1 week and 2 weeks after the nerve injury, F Groups RGCs number was significantly higher than other groups; PERG responses for all the treated groups were normal at 1 week after injury; however, at 2 weeks, only F groups remained so. Conclusion: Ultrasound-mediated BDNF gene transfection to the rat retina and visual cortex could significantly enhance the RGCs protection effect after optic Nerve Injury Key words: BDNF, retinal ganglion cells, visual cortex, gene therapy, ultrasound contrast agents 超声微泡介导BDNF基因联合转染大鼠视网膜和视皮质对于视神经损伤后神经节细胞的保护作用的实验研究 傅轶1，刘苏1，王志刚2，王小玲3，谢文跃1，王新宇1 1重庆医科大学附属第二医院眼科，重庆市 400010；2重庆医科大学超声影像学研究所，重庆市 400010；3重庆市女子职业高级中学，重庆市 400026 傅轶，男，1984年生，汉族，重庆人，在读硕士。主要从事青光眼方面的研究。E-mail：fuyi.doc@。联系电话通讯作者：刘苏，男，硕士，教授，重庆医科大学附属第二医院眼科，重庆市 400010。E-mail：liusu2836@。 国家自然科学基金重点项目 摘要 目的：利用超声微泡介导BDNF基因联合转染大鼠视网膜和视皮质区细胞，评估其对视神经损伤后视网膜神经节细胞（RGCs）的保护作用。 方法： SD雄性成年大鼠88只，随机分成6个组，分别为A正常组（8只）、B假手术组（16只）、C空白对照组（16只）、D眼转染组（16只）、E脑转染组（16只）、F联合转染组（16只）。A组不予处理，B组不钳夹视神经，余操作同C组，C-F组建立视神经钳夹伤模型，C组玻璃体腔和视皮质区注射PBS溶液；D组