大米镉结合蛋白的凝胶层析纯化及生化性质 - 食品安全质量检测学报.pdfVIP

6 6 Vol. 6 No. 6 2015 6 Journal of Food Safety and Quality Jun. , 2015 1 1, 2* 1 1, 2 1, 3 刘珊珊 , 陈季旺 , 陈 露 , 丁文平 , 吴永宁 (1. , 430023; 2. , 430023; 3. , 100021) 摘 要: 目的 (rice cadmium-binding protein, RCBP), RCBP 方法 Sephedex G-75 (Ethanol precipitation protein, EP) RCBP, SDSRCBP , FTIR RCBP 结果 b(RCBP), b RCBP, 32 kDa b 56.76% 19.41%, b N-H C=O , β-β-结论 RCBP 关键词: ; ; ; Purification and biochemical properties of cadmium binding protein from rice 1 1, 2* 1 1, 2 1, 3 LIU Shan-Shan , CHEN Ji-Wang , CHEN Lu , DING Wen-Ping , WU Yong-Ning (1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China; 2. Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan 430023, China; 3. National Center for Food Safety Risk Assessment , Beijing 100021, China) ABSTRACT: Objective To investigate the purification and biochemical properties of cadmium binding protein from rice and binding mechanism between cadmium and rice protein. Methods Rice cadmium-bining protein (RCBP) was purified from ethanol precipitation protein