不同底物和环境条件对两株铜绿假单胞菌接触烷烃方式及降解活性的影响.pdfVIP

应用与环境生物学报 2007，13(6)：843～847 Chin．，ApplEnviron Biol=ISSN1006．687X 不同底物和环境条件对两株铜绿假单胞菌 接触烷烃方式及降解活性的影响术 宋若海 2 华兆哲 2 陈坚 2 ( 江南大学教育部工业微生物重点实验室， 江南大学生物工程学院 江苏无锡 214036) 摘 要 考察了不同烷烃底物和温度、酸度、盐度等环境条件对两株铜绿假单胞菌接触烷烃方式和降解活性的影响． 结果表明，微生物接触烷烃的两种方式——直接接触方式和乳化接触方式同时存在于菌体降解烷烃过程中且受底物 和环境条件的影响．底物烷烃碳数的增加对菌株两种接触方式降解烷烃都有利，而盐度增加对它们都不利；高温、偏碱 性环境以及延长降解时间有利于菌体以乳化方式降解烷烃；低温、中性溶液环境有利于菌体以直接接触方式降解烷 烃．图4参12 关键词 铜绿假单胞菌；接触烷烃方式；烷烃降解活性；正烷烃；温度 CLC X172 Effects of Different Substrates and Environmental Factors on Hydrocarbon Accsess Mode and Degrading Ability of Two Pseudomonas aeruginosa Strains SONG Ruohai 一．HUA Zhaozhe CHEN Jian ， ( Key Laboratory ofIndustrial Biotechnology，Min~try ofEducation， School ofBiotechnology， Southern Yangtze University，Wuxi 214036，Jiangsu，China) Abstract Two Pseudomonas o,erugillOSCt strains，S and Y，were used here to study the effects of n—alkanes substrate and envi— ronmental conditions，including temperature，pH and salinity on microbial hydrocarbon access modes and degrading ability． The results showed that both microbial hydrocarbon access modes，that is，direct contact mode and biosurfactant—medium mode，existed in the petroleum hydrocarbon degradation process by the same strain and were affected by the substrate and envi— ronmental factors．It was found that the uptake of long—chain n—alkanes was improved by the two modes．but high salinity was disadvantageous to the alkane uptake process in either of the modes．High temperature，alkaline solution and long degradation time were advantageous to utilization of n—hexadecane substrate by biosurfactant—medium mode．while low temperature and mod— erate pH were more suitable for hydrocarbon biodegradation by direct contact mode．Fig 4，Ref 12 Keywords Pseudomonas aeruginosa；hydrocarbon ac