轮状病毒致胆道闭锁动物模型的建立.pdfVIP

中华小儿外科杂志2007年 12月第28卷第12期 Chin J Pediatr Surg，December 2007，Vo1．28，No．12 659 · 实 验 研 究 · 轮状病毒致胆道闭锁动物模型的建立 马亚贞 汤绍涛 童强松 王勇 毛永忠 李时望 董宁 阮庆兰 【摘要】 目的 应用猕猴轮状病毒(RRV)建立Balb／c小鼠胆道闭锁(BA)模型。方法 将新生 Balb／c鼠随机分为实验组和对照组，实验组腹腔注射猕猴轮状病毒液，对照组腹腔注射病毒培养液 (2％胎牛血清的最低必须培养基)，观察小鼠的临床表现及其组织病理学变化。结果 81只实验鼠 中63只(77．8 )鼠发生BA，出现发育迟缓(表现为体重增加缓慢、停滞)和胆汁淤积(表现为无毛区 黄疸、尿色深黄、陶土样大便)症状。大体观可见胆囊萎缩或扩张，肝外胆管呈索条状闭锁，组织连续 切片HE染色显示肝外胆管闭锁，与人类BA表现十分类似。21只对照鼠观察至21 d没有上述表 现。两组体重平均从第三天开始出现显著差异，在第九天差异达最明显，实验组为(3．65±0．19)g， 对照组为(5．96±0．25)g。结论 应用RRV能够建立稳定可靠的BA实验动物模型，为今后进行胆 道闭锁发病机制的研究提供了研究手段。 【关键词】 轮状病毒；胆道闭锁；模型，动物 Animal model of rhesus rotavirus induced biliary atresia induced MA Ya—zhen，7lANG Shao-tao， NGQiang—st；Jig，WANG Yong，MA0 Yong—zhong，LJ Shi—zoang，DONG Ning，R N Q ng-lan． Department¨，Pediatri( Surgery，Wuhan Union Hospital，Huazhong University of Science and ． T (。hnology。W “”430022，China Corresponding author：丁ANG Shao-tao，Email：tshaotao83@1 26．COD1 [Abstract] 0 ective To develop an animal modal 0f biliary atresia with the Rhesus Rotavirus (RRV)infection．Methods New born Balb／c mice were randomizeel into 2 groups．Group A mice were infected with rhesus rotavirus intraperitoneolly and group B were given 2 R 一MEM as contro1． The clinical and histological changes associateel with BA were observed and scored．The extrahepatic bile ducts werc serially sectioned and stained with hematoxylin and eosin．ResulIs Of the eighty-one mice from group A．sixty-three(77．8 )mice showed signs Of cholestasis and growth retardation． Hepatobiliary anatomy showed atrophy or dilation 0f gall bladder and extrahepatic biliary atrasia． These changes rcscmhlc biliary atresia in humans．None of the twenty one mice from group B developed any anomaly after jnoculation with MEM．Weight differ