特异性的蛋白小分子荧光探针及其标记技术 - 生命科学.pdf

20 1 Vol. 20, No. 1 2008 2 Chinese Bulletin of Life Sciences Feb., 2008 1004-0374(2008)01-0003-11 (200032) Q51; Q816 B Small-molecule fluorescent probes and their specific labeling protocols for proteins CHEN Lei, YAO Zhu-jun* (State Key Lab of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032,China) Abstract: Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in a variety of colors are useful tools for tracking expression and localization of fusion proteins in cells, these relative large tag can perturb protein folding, trafficking and function. Site-specific small-molecule fluorescent probe with advantages of small size, membrane permeability, low background and simple preparation will be an ideal tool in various studies of protein functions. This article summarizes the advances in selective labeling of proteins by using small-molecule fluo- rescent probes in recent years. Key words: protein; imaging; site-specific labeling; small-molecule fluorescent probe 1(GFP) (green fluo- rescent protein, GFP)[1](CFP, YFP ) [ 2 ] GFP 2007-10-29 2007-11-27 973(2002CB0713808); * E-mail: yaoz@mail.sioc.ac.cn 4