脑溢安对血红蛋白损伤神经元神经生长因子和白细胞介素1表达的影响.pdf

2001, 26( 1) 26 BULL HUNAN M D UNIV 1 贺双腾, 黎杏群, 张花先, 罗团连, 何泽云, 何 纲, 李家邦, 梁清华 中南大学湘雅医院中西医结合研究所(长沙 410008) [ ] 目的:观察脑溢安对体 培养神经元血红蛋白损伤后的保护作用及神经生长因子与白细胞介素1 表达 - 1 的影响方法: 以50mol L 血红蛋白造成培养海马神经元损伤, 运用活细胞计数Northern 杂交酶联免疫检测神经 元存活数量神经生长因子与白细胞介素1mRNA 及其蛋白表达水平结果: 体 培养大鼠海马神经元在含2% 牛血 - 1 清的培养液中可存活5 个月以上加入50mol L 血红蛋白培养24h 神经元死亡率为385% , 同时加入脑溢安药液 能显著降低神经元死亡率血红蛋白可引起培养神经元白细胞介素1mRNA 与蛋白表达水平显著升高及神经生长因 子的短暂升高,脑溢安能降低白细胞介素1 表达,维持神经生长因子持续表达结论: 脑溢安对血红蛋白损伤神经元 具有保护作用, 其机理与调节神经元白细胞素1神经生长因子表达有关 [ ] 神经生长因子; 白细胞介素1; 细胞,培养的; 脑溢安 [ ] R74334 [] A [ ] 2001 Effects of Naoyian on expressions of nerve growth factor and interleukin1 following injury induced by hemoglobin in cultured hippocampal neurons H Shuangteng, LI Xingqun, ZHANG Huaxian, et al Institute of Combined Traditional Chinese and Western Medicine , Xiangy a H osp ital , Central South University ( Changsha 410078 [Abstract] Objective: To investigate the effects of Naoyian (NYA) on protecting the cultured hippocam pal neurons against the injury induced by hemoglobin (Hb) and modulating the expressions of nerve growth factor (NGF) and interleukin1(IL1) following Hb injury. Methods: The experimental techniques of neuronal cul ture, alive cells count, Northern blotting, and enzymelinked immunosorbent assay ( LISA) were employed to detect the survival rate of cultured neurons, the expression level of NGF and IL1following Hb injury in cultured hippocampal neurons. Re