γ-氨基丁酸对情绪应激大鼠额叶皮质一氧化氮合酶和一氧化氮水平的影响.doc

γ-氨基丁酸对情绪应激大鼠额叶皮质一氧化氮合酶和一氧化氮水平的影响 欧阳俊彦，胡卓炎，褚 玥，梁 添，赖玉婷，羿利华，柳春红（510642 广州，华南农业大学食品学院，广东省食品质量安全重点实验室） [摘要] 目的 观察γ-氨基丁酸（GABA）对情绪应激大鼠一氧化氮合酶（NOS）和一氧化氮（NO）含量的影响，结合行为学实验，探讨γ-氨基丁酸抗焦虑作用机理。方法 将40只雄性Sprague-Dawley（SD）大鼠随机分为5组：对照组，模型组，GABA 0.5、1和2 mg/kg组，采用不确定性空瓶刺激的方法建立焦虑大鼠模型P＜0.01）；各组大鼠在中央区域的停留时间差异极为显著（P＜0.01）；对照组、GABA 1 mg/kg和2 mg/kg组进入中央区域的次数显著高于模型组（P＜0.05）；模型组的修饰次数显著高于GABA 1 mg/kg和2 mg/kg组（P＜0.05）；总运动距离和直立次数均无显著性差异（P＞P＜0.05）。结论 GABA与焦虑大鼠额叶皮质的一氧化氮合酶及一氧化氮均呈剂量-效应关系，提示GABA可能通过调节焦虑大鼠额叶皮质中的一氧化氮含量发挥抗焦虑作用。 [关键词] γ-氨基丁酸；焦虑；一氧化氮合酶；一氧化氮；旷场实验 [中图分类号] R749.4 [文献标志码] A Modulation of emotional stress-induced NOS and NO changes in frontal cortex by 4-aminobutyric acid in rats OuYang Junyan, Hu Zhuoyan, Chu Yue, Liang Tian, Lai Yuting, Yi Lihua, Liu Chunhong（College of Food Science，South China Agricultural University，The Key Laboratory of Food Quality and Safety，Guangzhou，510642，China） [Abstract] Objective To observe the effect of 4-aminobutyric acid(GABA) on NOS activity and NO levels combining behavioral test of rats exposed to emotional stress, and explore its anti-anxiety mechanisms. Method Utilizing the rat model of emotional stress induced by empty water bottles, 40 male Sprague-Dawley rats were divided into 5 groups randomly(n=8 each), control group, model group, GABA 0.5, 1 and 2 mg/kg group. Rats were pretreated with saline or different doses of GABA orally before they were suffered from emotional stress. 14 days after the treatment of GABA or saline with or without emotional stress challenge, animal behaviors were assessed in the open field test, NOS activity and NO levels of frontal cortex of each group were also determined. Results After the 14-day-emotional stress procedure, the food utilization of control group was significantly higher than other groups(P0.01); time spent at the center in the open field of each group was also significant different(P0.01). Meanwhile, significantly increased frequency in exploration towards to the center was seen