广州医科大学《临床免疫学检验》20.Autoimmune Diseases Immunoassays广医本科.pptVIP

临床检验专业 xiangliuhaiying@ Membranous pattern, also called Rim-like Pattern. The nucleolar pattern. Dot blot or line immunoassay are the popular methods testing a panel of autoantibodies. * ELISA is another popular method to detect ANA. It has advantages of standardization and automation. * RF is a kind of autoantibodies against human denatured IgG. IgM class RF was recognized as the main type of RF. A higher level of rheumatoid factor is closely associated with autoimmune disease, particularly rheumatoid arthritis. RF could be measured by radiaoimmunoassay, ELISA and immunoturbidimetry. RF is helpful in RA diagnosis , differential diagnosis and prognosis. In recent years, other ra-related autoantibodies have been identified, they were more specific than RF and were considered into the new score diagnostic system in 2009. ANCA is a group antibodies target the constituents of neutrophil cytoplasm. ANCA is the marker of systemic vasculitis. Also present in chronic inflammatory bowel disease and autoimmuen hepaptitis. Cytoplasmic ANCA 简称Canca. utrophil cytoplasm. ANCA relate with diseases of small -sized vasculitis, is the marker of systemic vasculitis. Also present in chronic inflammatory bowel disease and autoimmuen hepaptitis. Immune fluorescence assay is the routine method of ANCA, including three types of fluorescence patterns. Peripheral ANCA, 简称pANCA utrophil cytoplasm. ANCA relate with diseases of small