天津地区汉族人2型糖尿病与维生素D受体基因多态性的研究.pdfVIP

天津医药2008年4月第36卷第4期 255 天津地区汉族人2型糖尿病与维生素D受体 基因多态性的研究 张 鹏 苏文凌 沈保爽 李德强 邱明才 摘要 目的：探讨天津地区汉族人2型糖尿病(T2DM)与维生素D受体(VDR)基因多态性的关系。方法：采用聚 合酶链反应一限制性酶切片段长度多态性分析方法测定 116例T2DM患者和112例正常对照者的VDR一 1和 VDR—Bsml位点基因型，计算并比较T2DM患者和正常对照组基因型频率。结果：T2DM患者与正常对照组VDR— Fok1位点基因型、F，f等位基因的分布差异无统计学意义(P0．05)；VDR—Bsml位点基因型、S／b等位基因的分布差 异有统计学意义，T2DM患者VDR—Bsml位点Bb基因型、B等位基因明显增~II(P0．01)。结论：VDR基因Bsm1位 点Bb基因型、B等位基因可能是天津地区汉族人T2DM的基因型和易感基因。 关键词 糖尿病，2型 受体，骨化三醇 多态现象，遗传 聚合酶链反应 汉族 天津 Association between Vitamin D Receptor Gene Polymorphism and Type 2 Diabetes Mellitus of Han Nationality in Tianjin ZHANG Peng，SU Wenling，SHEN Baoshuang，LI Deqiang，Q1U Mingcai Department ofEndocrinology，TianjinMedical UniversityGeneralHospital，Tianjin300052，China Abstract Objective：To investigate the relationship between vitamin D receptor(VDR)gene polymorphism and type 2 diabetic mellitus(T2DM)in Han population in Tianjin．Methods：One hundred and sixteen patients with T2DM and 1 12 normal subjects as control were involved in this study．The VDR一 1 and VDR-Bsml genotypes were detected by polymerase chain reaction fragment length polymorphism(PCR-RFLP)in both groups．The allele frequencies and genotypic frequencies were calculated and compared between T2DM and normal controls．Results：There were no significant differences in allele ang genotype distributions ofVDR—Fok1 between T2DM and normal controls(P0．05)．However，the distributions of VDR—Bsml(B／b)genotype were increased in T2DM than that of normal control group(P0．01)． Conclusion：The VDR—Bsml B allele may be a susceptible gene contributing the development of T2DM in Han population inTianjin． Key words diabetic mellitus Type 2 receptors，caicitriol polymorphism，genetic polymerase