夏枯草水溶性多糖体外抗氧化活性研究 - 食品安全质量检测学报.pdf

5 6 Vol. 5 No. 6 2014 6 Journal of Food Safety and Quality Jun. , 2014 施晓丹, 张 霞, 殷军艺, 聂少平* (, 330047) 摘 要: 目的 方法 , (20%30%40%50%60%70%, v/v), (PVLP-1PVLP-2PVLP-3PVLP-4PVLP-5PVLP-6) (PVLP-2PVLP-3PVLP-4PVLP-5) DPPHO2- ··OH 结果 PVLP-2PVLP-3PVLP-4PVLP-5 DPPH , 3.2 mg/mL , 98.09%90.05%88.56% 87.71%O2- ··OH , PVLP-4 ·OH EC50 13.24 mg/mL , 10 mg/mL, PVLP-2 结论 , 关键词: ; ; ; Antioxidant activities of soluble polysaccharides from Prunella vulgaris Linn * SHI Xiao-Dan, ZHANG Xia, YIN Jun-Yi, NIE Shao-Ping (State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang 330047, China) ABSTRACT: Objective To investigate the antioxidant activities of soluble polysaccharides fractionated with ethanol. Methods Water soluble crude polysaccharide was extracted from Prunella vulgaris Linn with hot water and precipitated by ethanol, and subsequently separated by ethanol solutions with different concen- trations (20%, 30%, 40%, 50%, 60%, 70%, v/v) into six fractions (PVLP- 1, PVLP-2, PVLP-3, PVLP-4, PVLP-5, PVLP-6) with different molecular weights. The antioxidant activities of four fractions (PVLP-2, PVLP-3, PVLP-4, PVLP-5) were tested by determining