ox-ldl与单核巨噬细胞相互作用促进动脉粥样 - 中国实验动物学报.doc

研究报告 OX-LDL与单核巨噬细胞相互作用促进动脉粥样硬化形成 张 良, 韩 丹, 赵诗萌, 吴红敏 (中国医科大学附属第一医院新生儿科,沈阳 110001) 【摘要】 目的 单核-巨噬细胞在动脉粥样硬化（AS）发病过程中的作用日益受到关注，但泡沫化过程中细胞内脂质变化情况的研究报道尚不多见。方法 一次性密度梯度超速离心分离LDL制成ox-LDL，动态观察小鼠巨噬细胞内脂质成分和细胞形态的变化。结果 纯化的LDL纯度可达92.39%。细胞形态学观察细胞内红色脂质颗粒增多，电镜显示细胞核周围包含染色质，胞浆稀少含有大量的核糖体，线粒体丰富。随浓度的增加LDL组及OX-LDL组细胞内TC、FC、CE均明显增加。结论 OX-LDL同单核巨噬细胞相互作用可使动脉壁局部形成AS病变的特征病理性细胞。 OX-LDL较LDL更易使单核巨噬细胞形成泡沫细胞。高浓度的OX-LDL可以导致细胞膜结构的损伤。 【关键词】单核-巨噬细胞；动脉粥样硬化；LDL；OX-LDL；人；小鼠 【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2013.004.000 Interaction of OX-LDL and monocytes-macrophages promote atherogenesis ZHANG Liang, HAN Dan, ZHAO Shi-meng, WU Hong-min 【Abstract】Objective There is growing concern about the role of monocytes-macrophages in the progression of atherosclerosis (AS). However, the mechanism of lipid changes in those cells in the foaming process is not clear. Methods LDL was isolated by density-gradient centrifugation and to make Ox-LDL. The changes of lipid composition and cellular morphology of the rat macrophages were dynamically observed. Results The purity of separated LDL reached 92.39%. Increased amount of red lipid droplets in the macrophages were observed. Electron microscopy showed the presence of perinuclear chromatin, numerous mitochondria and ribosomes in the sparse cytoplasm. In the LDL group, TC, FC, and CE were all increased along with the increasing of LDL concentration. Similar results were observed in the Ox-LDL group. Conclusions Interaction of Ox-LDL and monocytes-macrophages may promote the formation of characteristic pathologic cells of AS nature in the arterial wall. OX-LDL is more easy than LDL to promote the changes of monocytes/macrophages into foam cells. High concentration of OX-LDL can lead to cell membrane damages. [Key words] Atherosclerosis; Monocytes; Macrophages; LDL; Ox-LDL; Human; Mouse ----------------------------------------------------------------------------------- [作者简介] 张