油菜油体钙蛋白基因bnclo1 的克隆和表达 - 作物学报.pdf

作物学报 ACTA AGRONOMICA SINICA 2008, 34(11): 1921−1928 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2008.01921 油菜油体钙蛋白基因BnClo1 的克隆和表达 丁 勇1,2 陈庆波1 徐春雷 1 常 玮3 甘 莉1,* (1 , 430070; 2 , 650224; 3 , 650204) : , RT-PCR RACE-PCR , 28.1 kD (caleosin)BnClo1 1 058 bp BnClo1 mRNA (GenBank AY966447) 3 Poly( A), DNA 6 5 Northern , BnClo1 , , , PCR , BnClo1 2 d , BnClo1 mRNA , , caleosin 245 (GenBank AAY40837), 3 N 1∼16 α-17∼61 N- ; 80∼120 C-N- Ca2+ EF-- (proline-knot) , 92∼114 α-, caleosin : ; RACE; ; ; ; Northern Cloning and Expression of BnClo1 Gene from Brassica napus DING Yong 1,2 , CHEN Qing-Bo 1, XU Chun-Lei 1 , CHANG Wei 3 , and GAN Li 1,* 1 2 ( National Key Laboratory of Crop Genetic Improement, Huazhong Agricultural University, Wuhan 430070, Hubei; School of Natural Resources, Southwest Forestry College, Kunming 650224, Yunnan; 3 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, Yunnan, China) Abstract: Triacylglycerols (TAGs) is stored in seeds as a nutrient for germination and postgerminative growth of seedlings. TAGs storage is confined to discrete spherical organelles called oil bodies. Plant seed oil bodies comprise a matrix of TAGs sur- rounded by a monolayer of phospholipids embedded with