液相色谱-离子阱质谱联用测定红花药材中金橙ⅱ染料 - 质谱学报.pdf

100 质谱学报 第28 卷增刊 液相色谱-离子阱质谱联用测定红花药材中金橙Ⅱ染料 1 1 1 1 1 2 金卫红 ，谭 力 ，张 玫 ，吴子刚，陆益红，顾峥嵘 （1. 江苏省药品检验所，江苏 南京 210008； 2. 江苏省常州药品检验所 江苏 常州 213001） Determination of Orange II Dye in Crocus sativus L. by HPLC-ESI-MS/ MS 1 1 1 1 1 2 JIN Wei-hong , TAN Li , ZHANG Mei , WU Zi-gang , LU Yi-hong , GU Zheng-rong (1.Jiangsu Institute for Drug Control, Nanjing 210008,China; 2.Changzhou Institute for Drug Control, Changzhou 213001,China) Abstract: A method for determination of Orange II Dye in Crocus sativus L. by reversed phase(RP) liquid chromatography-tandem mass spectrometry coupled with electro spray (LC-ESI-MS/ MS) was developed. The sample was extracted by water and analyzed directly upon concentration. An Agilent ZORBAX SB-C (250 mm 18 ×4.6 mm, 5 µm) column was developed to determine Orange II Dye in Crocus sativus L. samples. The mobile phase was a mixture solution of 0.15% formic acid and methanol. Mass spectrometry was operated in the negative ion mode. Ion fragments m/z 171 and m/z 247 were selected for qualitative analysis. The limit of detection (LOD) is 0.1 mg·L－1. The linear range is 0.5-20 mg·L－1. The average recovery is 100.2 %. The method is applicable for rapid routine monitoring. Key words : HPLC-ESI-MS/ MS ; orange II dye ; Crocus sativus L. 中图分类号：O657.63 文献标识码：A 文章编号：1004-2997（2007）增刊-100-03 金橙 II（2-萘酚偶氮对苯磺酸钠）为人工合成的酸性偶氮染料，也是一种酸碱指示剂。其结构 与苏