分子生物学复习题（Molecular biology）.doc

分子生物学复习题（Molecular biology） Molecular biology 1. Fill in the questions 1. The plasmid is classified by functional category with F plasmid, R plasmid and Col plasmid. (11) P10 - 2. Genetic diseases can be divided into single gene and polygenic diseases. (P36) 3. Molecular hybridization is composed of three steps: prehybridization, hybridization and elutriation. (P174) The laboratory must include four working areas of reagent storage and preparation; Specimen preparation area; Amplification reaction area; Product analysis area. (P129) In recombinant DNA technology, it is the restriction enzyme that is used by the DNA molecules of different sources. (P100) 6. The tumor is divided into three stages: priming stage, cancer stage and transformation stage. (P52) 7, biochip technology is based on the principle of specific interactions between biological molecules, such as DNA, DNA, DNA, RNA, antigen, antibody, can occur between receptor and ligand renaturation and specificity of combination, design one of the parties as the probe. (P193) 8. Nucleic acid molecular hybridization technology can be divided into isotopic hybridization and non - isotope hybridization according to the different markers of hybrid probes. (157). In the PCR reaction, the template includes genomic DNA, RNA, plasmid DNA and mitochondrial DNA. (P125) 10. DNA chip technology can be applied to gene diagnosis, DNA sequencing, clinical drug screening and other fields. (190). Causes of human prion disease are tremors, early dementia, GSS, and fatal familial insomnia. (P25) 12. The PCR reaction repeats the DNA replication process, and each replication involves three steps, namely degeneration, annealing (hybridization) and extension. The hydrogen bond rupture of DNA double helix is in the process of degeneration. (P125) The types of nucleic acid probes are DNA probes, RNA probes and oligonucleotide probes. (P166) The PCR reaction with RNA as the template is referred to as reverse transcription PCR. (P129) 15.