多波长快速测定巨鹿杏中有机酸和维生素含量及多地域杏产品比对研究.pdf

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.5 多波长快速测定巨鹿杏中有机酸和维生素含量 及多地域杏产品比对研究 1 2 1 1 1 1 刘峥颢 ，苏耀辉 ，牟霜 ，庞艳苹 ，李佩珊 ，魏聪聪 （1.河北大学质量技术监督学院，河北保定 071002）（2.河北金融学院，河北保定 071002) 摘要：本文采用高效液相色谱-二极管阵列检测器法（HPLC-DAD）快速检测杏中水溶性有机酸（苹果酸，柠檬酸和琥珀酸）和 维生素（维生素C、维生素B 、维生素B ）含量。以0.5 mmol/L 磷酸- 乙腈为流动相，随着时间推移改变流速和流动相比例，13 min 1 2 内梯度洗脱，采用多检测波长2 10 nm，254 nm 和270 nm 条件下，可使所测有机酸和维生素实现快速较好地分离。方法的稳定性在 0.75~2.12%范围内，精密度在0.94~1.55%之间，回收率测定结果为96.7~101.9%。采用本方法测定不同地域杏样品，提出巨鹿杏的特 征性品质。 关键词：多波长；有机酸；维生素；巨鹿杏 文章篇号：1673-9078(2013)5-1123-1127 Rapid Determination of Organic Acids and Vitamin in Ju’lu Apricot by a Multi-wavelength Analysis 1 2 1 1 1 1 LIU Zheng-hao , SU Yao-hui ,MU Shuang , PANG Yan-ping , LI Pei-shan , WEI Cong-cong (1.College of Quality Technical Supervision, Hebei University, Baoding, 071002, China) (2.Hebei Institute of Finance, Baoding, 071002, China) Abstract: High performance liquid chromatography (HPLC) with diode array detector (DAD) was applied to determine water -soluble organic acids (malic acid, citric acid and succinic acid) and vitamins (Vc, VB and VB ) in apricots sampled from Julu. The apricots samples 1 2 were eluted using 0.5 mmol/L H PO -acetonitrile as mobile phase at several wavelengths (210 nm, 254 nm and 270 nm) . The substances can be 3 4 efficiently separated in 13 min with RSD value ranged in 0.75~2.12%. The precisions were between 0.94% and 1.55% and the result