（graft NMR）Immobilization of Human Carbonic Anhydrase on Gold Nanoparticles Assembled onto Amine Thiol-Functionalized Mesoporous SBA-15 for Biomimetic Sequestration of CO2.pdfVIP

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（graft NMR）Immobilization of Human Carbonic Anhydrase on Gold Nanoparticles Assembled onto Amine Thiol-Functionalized Mesoporous SBA-15 for Biomimetic Sequestration of CO2.pdf

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（graft NMR）Immobilization of Human Carbonic Anhydrase on Gold Nanoparticles Assembled onto Amine Thiol-Functionalized Mesoporous SBA-15 for Biomimetic Sequestration of CO2

ARTICLE pubs.acs.org/Langmuir Immobilization of Human Carbonic Anhydrase on Gold Nanoparticles Assembled onto Amine/Thiol-Functionalized Mesoporous SBA-15 for Biomimetic Sequestration of CO2 Mari Vinoba,†,‡ Kyoung Soo Lim,† Si Hyun Lee,† Soon Kwan Jeong,*,† and Muthukaruppan Alagar‡ †Korea Institute of Energy Research, Daejeon 305-343, Korea ‡Department of Chemical Engineering, Anna University Chennai, Chennai 600-025, India ABSTRACT: A biocatalyst was synthesized by immobilizing human carbonic anhydrase onto gold nanoparticles assembled over amine/thiol-functionalized mesoporous SBA-15. The physicochemical properties of the functionalized mesoporous SBA-15 were obtained by XRD, BET, FE SEM, HR TEM, EDS, and zeta potential analysis. The biocatalytic performance was studied for para -nitrophenyl acetate (p-NPA) hydrolysis. The kinetic parameters Km were found to be 22.35 and 27.75 mM, and Kcat/Km values were 1514.09 and 1612.25 M1 s1 for HCA immobilized on gold nanoparticles assembled on amine/thiol-functionalized mesoporous SBA-15 (HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/ SBA-15), respectively. These HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/SBA-15 were investi- gated for biocatalytic hydration of CO and its precipitation as CaCO . The amount of CaCO 2 3 3 precipitated over HCA/Au/MPTES/SBA-15 was nearly the same as that precipitated over free HCA. Storage stability and reusability studies suggested that HCA/Au/MPTES/SBA-15 retained its activity even after 20 days storage at 25 C and 20 recycling runs. The present re