box-behnken 响应面法优选金樱子提取工艺 - 中国中医药信息杂志.pdf

2015 年8月第 22卷第8期 中国中医药信息杂志 ·99· Box-Behnken 响应面法优选金樱子提取工艺 江迎春,邓翀,李景丽 陕西中医药大学药学院,陕西 咸阳 712046 摘要：目的 筛选金樱子提取工艺。方法 以金樱子总皂苷、总酚酸含量为评价指标,乙醇浓度、提取时 间、料液比、提取次数为考察因素,采用 Box-Behnken 响应面设计,Design-Expert8.0.5 软件分析试验数据,建 立多元二次回归方程,筛选金樱子提取工艺参数。结果 筛选出金樱子的最佳提取工艺参数为：乙醇浓度 62.79%,料液比 13.12 ∶1,提取时间 137.93 min,提取次数 1.85 次,考虑到实际操作方便及工业大生产的实用 性,将提取工艺参数修订为乙醇浓度 60%,料液比 13 ∶1,提取时间 2 h,提取次数 2 次。结论 本研究筛选出的 参数可为金樱子的提取工艺提供依据。 关键词：金樱子；总皂苷；总酚酸；提取工艺；Box-Behnken 响应面法 DOI：10.3969/j.issn.1005-5304.2015.08.027 中图分类号：R283.5 文献标识码：A 文章编号：1005-5304(2015)08-0099-04 Optimization of Extraction Process of Effective Constituents from Rosae Laevigatae Frustus by Box-Behnken Response Surface Method JIANG Ying-chun, DENG Chong, LI Jing-li (School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, China) Abstract ：Objective To screen extraction process for Rosae Laevigatae Fructus. Methods With the content of total saponins and total phenolic acids from Rosae Laevigatae Fructus as the evaluation indexes, ethanol concentration, extraction time, solid-liquid ratio and extraction times as investigation factors, the test data were analyzed by Design-Expert8.0.5 software, and a multiple quadratic regression equation was set up to screen extraction process parameters of Rosae Laevigatae Fructus. Results The best extraction process parameters of Rosae Laevigatae Fructus were as follows ：ethanol concentration was 62.79%；solid-liquid ratio was 13.12 ∶1；the extraction time was 137.93 min；extraction times were 1.85 times. Considering the convenience for actual operation and the practicability of industrial production, the extraction process parameters should be ethanol concentration of 60%, revised solid-liquid rati