樱花多糖提取工艺的优化及抗氧化性研究 - 中国药事.pdf

58 中国药事 2015 年 1 月 第 29 卷 第 1 期 樱花多糖提取工艺的优化及抗氧化性研究 李友伟，温东东 ( 解放军第 401 医院，青岛 266071) 摘要 目的 ：通过正交试验优化樱花多糖的提取工艺，对樱花多糖的抗氧化功能进行了相关的研究， 为樱花多糖保健功能的研究提供试验依据。方法 ：通过正交设计优化樱花多糖的提取工艺，用蒽酮 - 硫 酸法测定不同提取条件下多糖的含量，并对其进行抗氧化性试验和自由基的清除试验。结果与结论 ：通 过对试验结果进行分析，最优工艺为 3 g 干燥的樱花花瓣置于烧杯中，加蒸馏水 240 mL，在 90 ℃的水浴 锅中提取 3 h，过滤，离心，得上清液。各种浓度的樱花多糖提取液均能够有效地清除超氧阴离子自由基 - -1 , (O2 ·), 且随着浓度的增大 其清除能力逐渐增强。浓度为 70.5 μg·L 多糖提取液的清除率为 58.82%。 关键词： 樱花多糖；正交实验；工艺优化；抗氧化 中图分类号：R965; R28 文献标识码：A 文章编号：1002-7777(2015)01-0058-05 Study on Optimization of Extraction Process of Cerasus Serrulata Polysaccharide and Its Antioxidant Activity Li Youwei , Wen Dongdong The 401th Hospital of PLA, Qingdao 266071 China) （ ， Abstract Objective: To optimize the extraction process of cerasus serrulata polysaccharide by the orthogonal experiment, conduct the related research of antioxidant function of cerasus serrulata polysaccharide and provide experimental basis for the research on the healthcare function of cerasus serrulata polysaccharide. Methods: The orthogonal design was used to optimize the extraction process of polysaccharide. The anthrone-sulfuric acid method was used to determine the content of polysaccharides in different extraction conditions and the oxidation resistance test and the free-radical clearance test were conducted. Results and Conclusion: Through the analysis of the test results, the optimum process was as follows. The 3 g dried Cerasus serrulata petals were put in the beaker with 240 mL distillated water and were extracted for 3 hours in the 90 ℃ water bath pot to obtain the supernatan