北京温榆河流域耐药大肠杆菌的调查研究 - 北京大学城市与环境学院.pdf

中国环境科学 2008,28(1) ：39~42 China Environmental Science 北京温榆河流域耐药大肠杆菌的调查研究 1,2 2* 2 2 2 1 施嘉琛 ,胡建英 ,常 红 ,万 祎 ,张照斌 ,相 艳 (1.北京航空航天大学环境工程系,北京100083；2. 北京大学城市与环境学院,北京 100871) 摘要：采用滤膜法结合大肠杆菌显色培养基,调查了北京地区温榆河流域氨苄青霉素、四环素、磺胺(磺胺甲厄唑-甲氧苄啶联用)和左旋氧 3 7 6 氟沙星4 类抗生素耐药大肠杆菌的发生情况.结果表明,温榆河流域总大肠杆菌数为10 ~10 个/L,耐药大肠杆菌数为0~10 个/L;大肠杆菌对 氨苄青霉素、四环素和磺胺耐药率分别为10%~35%、5%~25%、10%~35%,而使用时间最短的左氧氟沙星耐药率则低于 15%;受渔场排放 影响的采样点, 四环素耐药大肠杆菌数量显著高于该点其他抗生素耐药大肠杆菌数量,而左氧氟沙星耐药大肠杆菌数量百分比相对较高的 采样点推测可能源于人类排放. 关键词：耐药大肠杆菌；温榆河；抗生素；调查 中图分类号：X171.5 文献标识码：A 文章编号：1000-6923(2008)01-0039-04 Investigation on the antibiotic-resistance E.coli in Wenyu river in Beijing. SHI Jia-chen1,2, HU Jian-ying2*, CHANG 2 2 2 1 Hong , WAN Yi , ZHANG Zhao-bin , XIANG Yan (1.Department of Environmental Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100083, China；2.College of Environmental Science, Peking University, Beijing 100871, China). China Environmental Science, 2008, 28(1)：39~42 Abstract ：The occurrence of antibiotic-resistance E.coli to four classes of antibiotics including ampicillin, tetracycline, sulfonamide (sulfamethoxazole-trimethoprim) and levofloxacin in Wenyu river in Beijing was investigated by using membrane 3 7 filter method with E. coli chromogenic culture media. In Wenyu river basin, the total number of E. coli was 10 ~ 10 cell/L and the 6 number of antibiotic-resistance E. coli was 0~ 10 cell/L. The antibiotic-resistance rates to ampicillin, tetracycline, and sulfonamide were 10%~35%, 5%~25%, 10%~35% respectively; whil