七种中草药提取物抗肿瘤活性部位的筛选研究初报.pdf

2011,40(3): 31-35. Subtropical Plant Science 七种中草药提取物抗肿瘤活性部位的 筛选研究初报 郑志忠，陈良华，郑国华，童宇星，张树峰，童庆宣，明艳林 （厦门华侨亚热带植物引种园药用植物与植物药研发中心；厦门市植物引种检疫与植物源产物重点实验室，福建 厦门 361002 ） 摘 要：选取叶下珠、珠子草、千里光、鸦胆子、使君子、冬青和猫爪藤等 7 种中草药进行提取和初步分离， 筛选出有抗肿瘤效果的活性部位，并对其进行初步分析。采用 MTT 法，以 7 种中草药醇提物的不同分离部位 分别对人肝癌、胃癌、卵巢癌等肿瘤细胞株进行体外增殖抑制作用研究，并以人正常肝细胞株为模型对照跟踪 筛选活性部位。结果表明，从 7 种中草药醇提物中，均获得对肿瘤细胞株具有体外增殖抑制作用的化学分离部 位，其中叶下珠和珠子草的乙酸乙酯层和正丁醇层的活性最显著，千里光的乙酸乙酯层、鸦胆子的正丁醇层、 使君子和冬青的氯仿层活性次之，猫爪藤氯仿层和冬青的乙酸乙酯层活性较差，其他部位无活性。 关键词：中草药；MTT 法；筛选；抗肿瘤；活性部位 Doi: 10.3969/j.issn.1009-7791.2011.03.009 中图分类号：R931.71 文献标识码：A 文章编号：1009-7791(2011)03-0031-05 Preliminary Study on Screening Anti-Tumor Active Fraction of Extracts from Seven Chinese Herbal Medicines ZHENG Zhi-zhong, CHEN Liang-hua, ZHENG Guo-hua, TONG Yu-xing, ZhANG Shu-feng, TONG Qing-xuan, MING Yan-lin (The Research and Development Center for Medicine Plant and Plant Drugs, Xiamen Oversea Chinese Subtropical Plant Introduction Garden; Xiamen Botanical Plant Introduction, Quarantine and Plant Products Key Laboratory, Xiamen 361002, Fujian China) Abstract : Though extracting and separating on seven Chinese herbal medicines including Phyllanthus urinaria Linn ., Phyllanthus niruri Linn., Senecio scandens Buc h. -Ham. ex D. Don, Brucea javanica (Linn .) Merr, Quisqualis indica Linn., Ilex chinensis Sims and Macfadyena unguis-cati(Linn .)A.Gentry, the anti-tumor active fractions were screened, and their anti-tumor effects on different kinds of human tumor cell lines were preliminary analyzed. The proliferation inh ibitory effects in vitro of alcohol extracts on liver cancer, gastric cancer, oophoroma and