鱼卢鱼和小鼠肝组织几种功能指标的比较 - 上海海洋大学学报.pdf

第 16 卷第3 期 上海水产大学学报 Vo 1. 16 , No.3 2007 年5 月 JOURNAL OF SHANGHAI FISHERIES UNIVERSITY May ,2007 文章编号: 1004 - 7271 (2007) 03 - 0248 - 04 鱼卢鱼和小鼠肝组织几种功能指标的比较 杨彼珍，郁达义，李燕坤，吴旭干，成永旭 (上海水产大学农业部水产种质资源与养殖生态重点开放实验室，上海 200090) 摘 要:为探索处于不同进化地位的两种动物(萨鱼和小鼠)肝功能指标的异同，分别采用Lowry 法、CO 还原 差示光谱法和比色法，对自卢鱼和小鼠肝组织重要细胞器(线粒体和微粒体)蛋白、药物代谢酶(细胞色素 P450) 和反映肝功能的重要指标(血清中天冬氨酸转氨酶和丙氨酸氨基转移酶含量)进行了比较。结果显示， 自卢鱼肝脏线粒体蛋白、细胞色素P450 和丙氨酸氨基转移酶均明显低于小鼠，而微粒体蛋白和天冬氨酸氨基转 移酶与小鼠差异不明显。这些结果提示，肝功能指标在不同进化地位的动物(自卢鱼和小鼠)间存在着差异，其 中肝脏细胞色素 P450 和丙氨酸氨基转移酶的含量可能与肝功能差异密切相关。 关键词:自卢鱼;小鼠;肝;P450; 天冬氨酸转移酶;丙氨酸转氨酶 中图分类号:S 917 文献标识码:A Comparative study on functional indices of Iiver in largemouth bass (Micropterus salmoides) and mice YANG Xiao-zhen , YU Da-yi , LI Yan-wei , WU Xu-gan , CHENG Yong-xu (Key Laboratory 0/Aquatiε Genetic Resources αnd Aquaculture Ecosystem Certifìcated by the Ministry 0/Agriculture , Shanghai Fisheries University , Shanghai 200090 , China) Abstract: The important organelles protein (mitochondria and microsome) , cytochromes P450 of drug- metabolizi吨 enzymes as well as aspartate transaminase ( AST) and alanine transaminase ( ALT) in serum of important liver functional indices were comparatively analyzed by Iρwry protein measure , CO difference spectrum and colorimetric method to explore the difference of liver functional indices between largemouth bass and mice from different evolution status. Results showed that the level of mitochondria protein , cytochromes P450 as well as ALT in largemouth bass liver were significantly lower compared to mice. The level of microsome protein and AST in lar