竹加工剩余物制备乙醇联产低聚木糖的研究 - 南京林业大学学报.doc

竹加工废弃物制备低聚木糖和乙醇 李寒李鑫陈牧余世袁勇强* （南京林业大学化学工程学院，江苏 南京，210037） 摘要：开展了竹加工废弃物综合利用制备低聚木糖和乙醇的研究。内切木聚糖酶定向降解竹屑木聚糖制备高附加值产品低聚木糖，木聚糖抽提剩余物经糖化、发酵制备乙醇。7%的NaOH提取竹屑木聚糖1 h，木聚糖提取率57.81%。用量为25 IU/g木聚糖的内切木聚糖酶定向水解木聚糖，低聚木糖得率39.49%，低聚木糖对青春双歧杆菌有良好的增殖作用。用量分别为25FP IU/g纤维素和4IU/g纤维素的纤维素酶和β?葡萄糖苷酶水解木聚糖抽提剩余物72 h，纤维素酶水解得率64.05%。葡萄糖浓度为g/L的纤维素水解糖液经酿酒酵母NLH13发酵40 h，糖利用率和乙醇得率分别为98.0%和%。 关键词：竹加工剩余物；低聚木糖；乙醇；内切木聚糖酶；纤维素酶 Xylo-oligosaccharides and ethanol production from bamboo processing residues LI Han, LI Xin, XU Yong, CHEN Mu, YU Shi-yuan, YONG Qiang* （College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China） Abstract:ylo-oligosaccharides and ethanol preparation from bamboo processing residues were investigated. The bamboo xylan was selected degraded by endo xylanase to produce xylo-oligosaccharides, xylan extraction residue was used for ethanol production through saccharification and fermentation processes. The xylan of bamboo processing residues was extracted with 7% NaOH solution for 1h, the xylan extraction yield was 57.81%. The xylan was hydrolyzed by endo xylanase with the dosage of 25IU/g xylan for 12h, the xylo-oligosaccharides yield was 39.49%, and the xylo-oligosaccharides have good effect on the proliferation of Bifidobacterium adolescentis. The xylan extraction residue was hydrolyzed by cellulase and β-glucosidase with the dosage of 25FPIU/g cellulose and 4IU/g cellulose for 72h, 64.05% hydrolysis yield was obtained. The hydrolyzate containing 121.20g/L glucose was fermented by Saccharomyces cerevisiae NLH13 for 40h, the glucose consumption and ethanol yield were 98.80% and 92.95%, respectively. Keywords: Bamboo processing residues; Xylo-oligosaccharides; ethanol; endo xylanase; cellulase  我国是竹资源大国，竹林面积万，占全国森林面积的%[1]。竹子是典型的生态经济型植物，近年来我国竹材加工产业呈快速发展态势，竹子被广泛用来替代木材生产竹胶合板、竹地板、竹家具和生产竹纤维替代化纤产品等[2-3]。但我国竹加工产业尤其是竹板型材生产原料利用率低，仅为原竹的30~50%，加工过程中产生大量竹屑废弃物，通常作为一般燃料使用，经济价值低。竹是富含纤维素和的废弃物资源[4]。采用生物技术对这些废弃物资源精深加工，可生产高附加值糖工程产品、生物能源和大宗化学品，不仅可以提高资源利用率和经济价值，而且对于缓解我国能源短缺问题、节能减排和发展农村经济有重要意义