白腹皮蠹幼虫肠道细菌分离及鉴定 - 安徽农业大学.pdfVIP

安徽农业大学学报, 2013, 40(3): 482-486 Journal of Anhui Agricultural University 网络出版时间：2013-4-26 15:37:13 [URL] /kcms/detail/34.1162.S1537.019.html 白腹皮蠹幼虫肠道细菌分离及鉴定 * 秦 浩，李志鹏，李林懋，刘玉升 ，郑继法 ( 山东农业大学植物保护学院，泰安271018) 摘 要：为了研究白腹皮蠹幼虫肠道环境中的细菌种类，从中分离、纯化、培养，获得 6 个细菌菌株，对其培 养性状、染色反应、菌体形态、生理生化反应进行系统研究。鉴定结果表明，上述 6 个细菌菌株分别属于坚强芽孢 杆菌（Bacillus firmus ）、气微菌属（Aeromicrobium Miller, Woese and Brenner, 1978 ）、浸麻芽孢杆菌（Bacillus macerans) 、葡萄球菌属（Staphylococcus Rosenback,1884 ）、巨大芽孢杆菌（Bacillus megaterium ）和短芽孢杆菌（Bacillus brevis ）。从白腹皮蠹肠道环境中分离出 6 个菌株， 鉴定出分类地位， 菌株之间的数量存在明显差异，以巨大芽孢 杆菌数量最多（5.32 ×1019 ），需要进一步研究该6 个菌株在白腹皮蠹肠道环境中的具体作用，以期寻求有效防治白 腹皮蠹的新途径。 关键词：白腹皮蠹；肠道细菌；分离；鉴定 中图分类号：S433.5 文献标识码：A 文章编号：1672−352X (2013)03−0482−05 Isolation and identification of intestinal bacterial flora of Dermestes Maculatus Degeer larvae QIN Hao，LI Zhi-peng ，LI Lin-mao ，LIU Yu-sheng，ZHENG Ji-fa (College of Plant Protection, Shandong Agricultural University, Tai’an 271018) Abstract: In order to study the bacterial population of the larvae of Dermestes Maculatus Degeer, six kinds of bacteria were isolated from the larvae of Dermestes Maculatus Degeer after separation and purification. In ad- dition, the bacterial modality, dyeing reaction, cultural character and physiological and biochemical reactions were studied systematically. The identification results showed that the six bacteria belong to Bacillus firmus, Aeromi- crobium, Bacillus macerans, Staphylococcus, Bacillus megaterium and Bacillus brevis, respectively. The signifi- cant differences among the counts of the 6 strains isolated from the larvae of Dermestes Maculatus Degeer were 19 observed, and Bacillu