（sprattus sprattus）仔鱼sod和cat酶活性明显较高 - 中国实验动物学报.docVIP

研究报告 水基钻井液对诸氏鲻虾虎鱼抗氧化酶的影响 蔡文超，陈小曲，李建军，黄韧 （广东省实验动物重点实验室 广东省实验动物监测所 广州 510663） [摘要]目的 研究我国南海区某海洋钻井水基钻井液对诸氏鲻虾虎鱼抗氧化酶的影响。 方法 在19.75～158 mg/L浓度下，对3月龄诸氏鲻虾虎鱼染毒，并用试剂盒法测定SOD和CAT的活性。 结果 SOD对水基钻井液的毒性暴露不太敏感，在染毒第1 天只有最低浓度组受到一定程度的抑制，染毒第2 天各浓度组酶活性均受到一定程度的诱导（12.4%～25.5%），这种诱导作用在第4 天即有所减弱，之后酶活性的变化变缓。CAT则对水基钻井液表现出较强的敏感性。染毒第1 天，各组酶活性均受到明显的诱导，并且各组酶活性表现出明显的浓度效应关系。最高浓度组酶活性最大诱导率达到97%（P0.05）。随后虾虎鱼CAT酶活性逐渐下降，第7 天，各染毒组酶活性的抑制率达到58.4%～89.1%。 结论 CAT在中毒反应中表现出了敏感性，具备了作为生物标志物的重要条件，有望在今后的实际应用中发挥作用。[关键词] 水基钻井液，诸氏鲻虾虎鱼，生物毒性，SOD，CAT 【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2014.002.000 Effect of waterbased drilling fluid exposure on the activity of antioxidant enzymes in Mugilogobius chulae CAI Wen-chao, CHEN Xiao-qu, LI Jian-jun, HUANG Ren (Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animal Monitoring Institute, Guangzhou 510663, China) [Abstract] Objective To study the effect of waterbased drilling fluid (from a drilling platform in Nanhai district, China) exposure on the activity of antioxidant enzyme in Mugilogobius chulae. Methods The SOD and CAT activity of 3-month-old juveniles were tested by reagent kit method during the exposure at 19.75 to 158 mg/L concentration. Results The SOD activity of 3-month-old juveniles was not sensitive during the exposure. At the first day of exposure, the SOD activity was slightly inhibited at the lowest concentration (P0.05). However, the activity was inducted (12.4 % to 25.5 %, P0.05) in all treatment at the second day. During the 7-day exposure, the change of SOD activity was limited. The activity of CAT showed high sensitivity to the drilling fluid. During the first day of exposure, the CAT activities of each treatment were inducted and they had a concentration effect. The inductivity of the highest concentration even reached to 97% (P 0.05). Then, the inductivity slowed down to 58.4% to 89.1% at the