原创力文档Application of atmospheric pressure ionization HPLC-MS-MS for the analysis of natural products：（应用程序的大气压电离HPLC-MS-MS天然产物的分析）.pdfVIP
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Application of Atmospheric Pressure Ionization HPLC-MS-MS
for the Analysis of Natural Products
M. Herderich / E. Richling / R. Roscher / C. Schneider / W. Schwab / H.-U. H u m p f / P. Schreier*
Lehrstuhl ftir Lebensmittelchemie, Universit~it WUrzburg, Am Hubland, 97074 Wtirzburg, Germany
Introduction
Key Words
Column liquid chromatography The combining of chromatographic and spectroscopic
Tandem mass spectrometry (MS-MS) information is a well known strategy; a classical example
Electrospray ionization (ESI) is the coupling of gas chromatography with mass
Atmospheric pressure chemical ionization (APCI) spectrometry for the analysis of volatile compounds or
Selected reaction monitoring (SRM) substances which can be transformed to sufficiently
Natural products volatile derivatives. For the analysis of polar com-
pounds, combining HPLC with mass spectrometry
should be the method of choice. In order to transform
Summary molecules from solution to ions in the gas phase, three
major problems of incompatibility must, however, be
The development of techniques utilizing atmospheric overcome:
pressure ionization, namely atmospheric pressure
(i) the apparent problem of maintaining the high
chemical ionization (APCI) and elect
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