有机酸在合成培养基中对木醋杆菌合成纤维素的影响-食品科学.PDF

※生物工程 食品科学 2008, Vol. 29, No. 05 295 有机酸在合成培养基中对木醋杆菌 合成纤维素的影响 王志国，韦燕来，韦茂山，王锡彬，向 东 (海南大学 海南省热带水生生物技术重点实验室，海南 海口 570228) 摘 要：在合成培养基中分别添加0.1% 柠檬酸、苹果酸、丁二酸、乳酸、乙酸、丙酮酸，发现只有乳酸、 乙酸、丙酮酸能使木醋杆菌在合成培养基中繁殖且产生纤维素。当0.4% 丙酮酸加入时，纤维素产量最高，达到2. 02g/L；0.3%乳酸加入时，纤维素产量最高，达到 2.16g/L；0.4% 乙酸加入时，维素产量最高，达到2.80g/L。当 0.4% 乙酸、0.3% 乳酸、0.4% 丙酮酸同时加入，纤维素产量可达4.45g/L。 关键词：木醋杆菌；合成培养基；有机酸 Effect of Organic Acids on Bacterial Cellulose Production in Defined Medium by Acetobater xylinum WANG Zhi-guo，WEI Yan-lai，WEI Mao-shan，WANG Xi-bin，XIANG Dong (Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, Hainan University, Haikou 570228, China) Abstract ：0.1% citric acid, malic acid, succinic acid, lactic acid, acetic acid, pyruvic acid was added to the defined medium to study their effects on cellulose production individually. The results showed that only lactic acid, acetic acid, pyruvic acid among Acetobacter xylinum and enhance cellulose yield.When 0.4 % pyruvic acid added, yeild the above organic acids could proliferate of cellulose was highest 2.02 g/L; When 0.3% lactic acid was added, yeild of cellulose was highest 2.16 g/L; When 0.4 % acetic was acid added, yeild of cellulose was highest 2.80 g/L; When 0.4% acetic acid, 0.3%lactic acid and 0.4% pyruvic acid coexisted in the medium, the yeild was up to 4.45 g/L. Acetobacter xylinum；organic acids；defined medium Key words： 中图分类号：Q815 文献标识码：A 文章编号：1002-6630(2008)05-0295-03 目前，在国内，广泛运用于食品中的细菌纤维素 究所鉴定)。 主要通过自然发酵椰子水生产。而原料的季节性及自然 1.1.2 培养基 发酵椰子水成分的复杂性，使细菌纤维素的商业化生产 种子液培养基：2% 葡萄糖