连翘对水貂呕吐模型的止吐作用和其止吐机制研究.pdfVIP

提 要 目的：利用水貂呕吐模型进一步研究连翘的止吐作用并探讨其止吐机制。 方法：（1）整体实验：分别利用顺铂、阿朴吗啡、硫酸铜制备水貂呕吐模型。 将水貂随机分为模型组、阳性药对照组和连翘治疗组。观察各组动物在给予顺 铂、阿朴吗啡、硫酸铜后 6h 内的反应，记录呕吐发生的潜伏期、干呕和呕吐次 数。（2 ）离体实验：以呕吐相关受体激动药为工具药，利用离体恒温浴槽，观 察连翘对豚鼠离体回肠运动的影响。结果：（1）连翘能够延长顺铂、阿朴吗 啡、硫酸铜所致的水貂呕吐潜伏期，减少干呕次数。（2 ）连翘水煎剂均能显著 抑制豚鼠离体回肠的自发活动，降低其收缩张力，且呈剂量依赖性关系。连翘 -1 -1 -1 大、中、小三个剂量（10mg·mL ，5mg·mL ，2mg·mL ）均能抑制乙酰胆 碱、组织胺、五羟色胺所导致的肠管收缩，降低收缩张力和振幅，但对收缩频 率没有明显影响。连翘大剂量和中剂量能够拮抗多巴胺引起的肠管收缩松弛， 使张力和振幅变大，小剂量能够进一步降低由多巴胺所致的肠平滑肌收缩平均 张力，但能够增大肠平滑肌收缩振幅。结论：（1） 连翘对上述三种经典催吐 剂导致的水貂呕吐模型均具有止吐作用。其机制可能通过中枢和外周多重途径 而发挥止吐作用。（2 ）连翘可以抑制豚鼠回肠运动，其机制可能是通过阻断肠 平滑肌上的 M 受体、H1 受体、5-HT 受体和 D2 受体，也可能是对肠管的直接抑 制作用，其止吐机制尚待进一步深入研究。 关键词 连翘；止吐；水貂；豚鼠；回肠 Anti-emetic effects of Lianqiao on a Emetic Model in Mink and its mechanism studies in ileum in guinea pig Speciality: Basic Research of Integrated TCM and Western Medicine Author: Ma Hongxin Supervisor: Prof. Nie Ke Abstract Objective: To investigate the anti-emetic effects of Lianqiao(LQ) and analyze the mechanism of anti-emetic effects of LQ. Methods: (1) Established nausea model of minks induced by cisplatin, apomorphine and copper sulfate. Divided the minks into model group, control group and treatment group randomly. Observing and recording reaction of the minks, the potential period of vomitting, the number of retching and vomiting after they were given cisplatin, apomorphine and copper sulfate in the subsequently six hours. (2) Using emesis-relating agonists as drugs, the inhibitory effects of LQ on guinea pig ileum contractility in vitro were observed in organ bath. Results: (1) LQ can prolong the potential period of the vomitting and decredse the number of retches induced by cisplatin, apomorphine and copper sulfate. (2) W