ri基因真核表达载体的构建及其对huvec增殖的 - 第三军医大学学报.doc

过表达RI对膀胱癌移植瘤生长的影响 ) [摘要] 目的 探讨(ribonuclease inhibitor，RI)基因对膀胱癌裸鼠移植瘤生长及表达的影响实验分3组细胞组)、(转染阴性对照质粒的)、(正常) 。将pcDNA3.1RI、pcDNA3.1–转染人膀胱癌,筛选稳定表达,将其注入裸鼠皮下,检测裸鼠移植瘤的大小和质量,HE染色观察移植瘤组织的形态学变化,利用免疫组化技术检测移植瘤组织中微血管的变化 组较与,瘤体质量显著降低,抑瘤率分别为.25%和6.13%。组癌细胞较与组体积减小,组微血管较与组明显减少甚至无血管出现。RI，可以抑制血管生长,能抑制膀胱癌裸鼠移植瘤的生长,并通过降低ANG的表达抑制膀胱癌裸鼠移植瘤的增殖膀胱癌Li Hongyan , Pan Xiangyang,Yao Xue, Xiong Dongmei, Chen Junxia（Clinical laboratory,Nanchong central hospital ,Sichuan, 637000，China） [Abstract] Objective To study over-expressing of RI on the growth of bladder cancer and the mechanism.Methods Cells of each group (T24, T24 vector, T24-RI) were collected and injected into the backs of the BALB/C nude mice,five weeks after injection, the mice were sacrificed and the tumors were removed and weighted. The micorvessels of tumor and whether the cancer cell have metastasized to lung were detected by HE dying. At the same time, immunofluorescence assay of CD31 was executed to determine the influence of RI on new blood vessel formation. Results The results showed that the T24-RI cells group significantly inhibited the growth of bladder cancer compared with the other two control groups. Immunofluorescence assay of CD31 and HE stain was executed. Results showed that T24-RI cell groups revealed aless microvessels in tumor tissue than two other control groups. Mice injected with the T24-RI cell groups also showed a remarkable suppression of the spontaneous lung metastasis, in which no metastasis was observed in T24-RI cell groups. Conclusion The eukaryotic expressive vector of RI gene can inhibited the growth of bladder cancer significantly. [Key words] ribonuclease inhibitor; Bladder cancer cells; eukaryotic expression Supported by the General Program of National Natural Science Foundation of China. [基金项目] 国家自然科学基金面上项目 膀胱癌是世界第二高发性生殖泌尿系统恶性肿瘤，尽管它的病发率只占有我们所发现的前列腺癌的四分子一，它高复发率及在治疗与现有水平的诊断过程中困难使得膀胱癌成为目前最为昂贵的治疗[1]。人核糖核酸酶抑制因子(Human ribonuclease inhibitor，RI)是一个分子量为50kD的酸性蛋白，它具有7个亮氨酸残疾重复片段组成的亮氨酸拉链