硬骨鱼类卵黄原蛋白及其在卵子发生中的作用 - 中国细胞生物学学报.pdf

网络出版时间：2017-04-11 10:34:27 网络出版地址：/kcms/detail/31.2035.Q1034.010.html 中国细胞生物学学报 Chinese Journal of Cell Biology 2017, 39(5): DOI: 10.11844/cj cb.2017.05.0266 硬骨鱼类卵黄原蛋白及其在卵子发生中的作用 周 阳 侯聪聪 竺俊全* (宁波大学教育部应用海洋生物技术重点实验室, 浙江省海洋高效健康养殖协同创新中心, 宁波3152 11) 摘要 卵黄原蛋白(vitellogenin, Vtg)作为卵黄蛋白(yolk protein, YP)的前体, 参与卵生动物的卵子 发生。硬骨鱼类的Vtg为同二聚体, 存在不同的亚型, Vtg单体(monomer) 由5个结构域组成。在硬骨鱼 类卵子发生过程中Vtg主要有两方面作用: (1)Vtg被卵母细胞吸收后裂解为卵黄蛋白, 贮存在卵母细胞 中, 为其生长发育提供必需的营养; (2)随着卵母细胞的发育, 卵黄蛋白裂解为游离氨基酸, 调节卵母细 胞的渗透压, 保证水合作用的顺利进行。该文介绍了硬骨鱼类Vtg的种类及结构特性, 并对Vtg的表达、 吸收、分解及其参与水合作用的研究进展作简要综述, 以期为硬骨鱼类卵子发生机制的研究提供参考。 关键词 硬骨鱼类; 卵黄原蛋白; 卵黄发生; 水合作用; 渗透压 The Vitellogenin and Its Function during Oogenesis in Teleost Fishs Zhou Yang, Hou Congcong, Zhu Junquan* (Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Zhejiang Province Collaborative Innovation Center of Marine Efficient and Healthy Aquaculture, Ningbo 315211, China) Abstract Vitellogenin (Vtg), as the precursor of yolk protein (YP), involved in the oogenesis in oviparous animals. The structure of teleost fishes’ Vtg is the homodimer and present different subtypes. The Vtg monomer is consist of five different domains. During the oogenesis in teleost, the Vtg plays two functions. On the one hand, the Vtg is hydrolyzed into yolk protein and store in the oocyte to provide the necessary nutrition for the oocyte development. On the other hand, the yolk protein is hydrolyzed into free amino acid with the develo