1 血清specam-1水平与糖尿病肾病的相关性研究 陈秀锋 王新华 胡静 .docVIP

血清sPECAM-1水平与糖尿病肾病的相关性研究 摘要 目的：血清血小板内皮细胞黏附分子-1（Platelet endothelial cell adhesion molecule-1, PECAM-1）与糖尿病肾病（diabetic nephropathy）的相关性，探讨可溶性PECAM-1（sPECAM-1）能否成为反映糖尿病肾病病程进展的生物标志物。方法：选取2013年7月至2015年6月我院2型糖尿病住院病人156例。根据尿白蛋白排泄率（urinary albumin excretion rate, UAER），将糖尿病患者分为正常白蛋白尿组（UAER20 μg/min）55例，微量白蛋白尿组（20 μg/minUAER200 μg/min）48例，大量白蛋白尿组（糖尿病肾病组）（UAER200 μg/min）53例。选择同期30例门诊年龄、性别相匹配的正常体检者为对照组。对住院病人采集病史，收集相关实验室检查结果。采用酶联免疫吸附法（ELISA）定量测定血清中sPECAM-1含量。结果：糖尿病微量白蛋白尿组（67.1±8.3 ng/ml）和大量白蛋白尿组（79.1±7.5 ng/ml）血清sPECAM-1水平显著高于对照组（51.0±4.9 ng/ml）和正常白蛋白尿组（53.6±5.7 ng/ml），而且大量白蛋白尿组的血清sPECAM-1又显著高于微量白蛋白尿组（P0.05）。糖尿病患者血清sPECAM-1与相关参数进行Pearson相关分析显示，sPECAM-1与 收缩压，舒张压，空腹血糖，TG，血尿素氮，血清肌酐和尿白蛋白排泄率（UAER）呈正相关. 多变量分析中显示sPECAM-1与糖尿病肾病预后。结论：2型糖尿病患者血清sPECAM-1显著升高，且随患者尿白蛋白增加而增加。血清sPECAM-1与糖尿病患者的肾脏损害相关，可以作为预测糖尿病肾病发展及病情严重程度生物学标志之一。 关键词：2型糖尿病；糖尿病肾病；sPECAM-1；肾功能损害 Correlation between serum sPECAM-1 level and diabetic nephropathy CHEN Xiufeng, WANG Xinhua, HU Jing, CHEN Jie, LIAO Lin, HAN Haiyan, ZHANG Chuanfu, LI Wufeng, LI Lulu, LIU Wenrui, LU Jianrao Abstract Objective: To investigate the correlation between serum platelet endothelial cell adhesion molecule - 1 (PECAM-1) and diabetic nephropathy (DN), and to explore the potential of soluble PECAM-1 (sPECAM-1) as a biological marker for early detection and progression monitoring of diabetic nephropathy. Methods: A total of 156 hospitalized patients with type 2 diabetes mellitus were included from July 2013 to June 2015. According to urinary albumin excretion rate (UAER), the diabetic patients were divided into normoalbuminuria group (UAER 20 μg/min) of 55 cases, microalbuminuria group (20 μg/min UAER 200 μg/min) of 48 cases, and macroalbuminuria group (diabetic nephropathy group) (UAER 200 μg/min) of 53 patients. The control group was selected from 30 healthy subjects matched for age and sex. The medical history and relevant laboratory results were collected from each patient. Serum sPECAM-1 level was determined by enzyme-linked immunosorbent