蓝莓多酚对巨噬细胞中iNOS、COX-2 基因表达的影响.PDF

144 2014, Vol.35, No.19 食品科学 ※生物工程 蓝莓多酚对巨噬细胞中iNOS 、COX-2 基因表达的影响 1,2 1,* 1 1 2 韩彩静 ，程安玮 ，陈相艳，王文亮，谢春阳 （1.山东省农业科学院农产品研究所，山东 济南 250 100 ；2.吉林农业大学食品科学与工程学院，吉林 长春 130 118 ） 摘  要：RAW264.7 巨噬细胞被脂多糖预刺激后，添加从蓝莓中分离制备的不同质量浓度的可萃取多酚（extractable polyphenols，EPP ）和不可萃取多酚（non-extractable polyphenols，NEPP ），培养不同的时间，研究不同质量浓 度及培养时间对诱导型一氧化氮合酶（inducible nitric oxide synthase ，iNOS ）、环氧合酶（cyclooxygenase-2 ， COX-2 ）基因相对表达量的影响。结果表明：从蓝莓中分离的EPP和NEPP均能抑制iNOS、COX-2基因表达，在12 h 培养时间内，2 种多酚的抑制效果较好，且EPP 的抑制效果明显好于NEPP 。这证明蓝莓中的NEPP 同EPP一样，可 通过抑制iNOS、COX-2 mRNA 的表达表现出明显的抗炎活性。 关键词：蓝莓多酚；巨噬细胞；一氧化氮合酶；环氧合酶；基因表达 Effects of Blueberry Polyphenols on the Expression of iNOS and COX-2 Genes in Macrophages 1,2 1,* 1 1 2 HAN Cai-j ing , CHENG An-wei , CHEN Xiang-yan , WANG Wen-liang , XIE Chun-yang (1. Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250 100, China; 2. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130 118, China) Abstract: The effects of different concentrations of extractable polyphenols (EPP) and non-extractable polyphenols (NEPP) from blueberries on the mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharides (LPS)-induced RAW264.7 cells at different culture times were studied in this paper. The results showed that both EPP and NEPP from blueberries inhibited the mRNA expression of iNOS and COX-2 genes. During a 12-h incubation period, the inhibitory effects of both EPP and NEPP, especially that of EPP, were significant. It is suggested that EPP a