强志组方对多发性抽动症复合恐惧行为大鼠的影响 - 中国实验动物学报.doc

研究报告 强志组方复合恐惧大鼠的影响 山东中医药大学，济南 250000；2. 山东中医药大学附属医院，摘要目的 探究强志组方治疗多发性抽动症（TS）复合恐惧行为的作用机制。方法 腹腔注射亚氨基二丙腈（IDPN）建立TS大鼠模型后，进一步声电刺激建立TS复合恐惧大鼠模型，给予不同药物灌胃治疗后，采用旷场试验、行为学检测大鼠行为变化，高效液相法（HPLC）检测脑组织中多巴胺（DA的含量，免疫组织化学法检测酪氨酸羟化酶（TH）的含量，RT-PCR检测TH mRNA 的表达。结果 与正常对照组相比，模型对照组刻板行为、运动行为增加，冻结时间延长，脑组织中 DA、TH、TH mRNA 无明显变化；与模型对照组相比，强志组方组刻板行为、运动行为减少，冻结时间，脑组织中 DA 、TH、TH mRNA 含量减少。结论 强志组方可改善模型大鼠抽动复合恐惧行为，其作用机制可能是通过下调 TH mRNA表达，降低 TH 含量，减少 DA 合成实现的。 关键词强志组方多发性抽动症恐惧行为多巴胺酪氨酸羟化酶 【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2016.02.000 Effect of “Qiangzhizufang” on the rat model of Tourette syndrome combined with fear JIN Zhi1, YAN Zhao-jun2, LI Ya-qun1, ZHAO Xing-you1, WU Jin-yong2, XI Lei-ming2 (1. Shandong University of TCM, Jinan 250014, China;2. Affiliated?Hospital of Shandong University of TCM, Jinan 250014) 【Abstract】Objective To explore the functional mechanism of a Chinese medicine compound “qiangzhizufang” on rat model of Tourette syndrome combined with fear. Methods The rat model of TS combined with fear was established by intraperitoneal injection of 3,3’-iminodipropionitrile (IDPN) combined with acoustic stimulation. After giving different drug lavage treatment, the changes of behavior of the rat models were assessed by field test and behavior test. The content of DA, TH and TH mRNA in the brain tissue was detected by HPLC, immunohistochemistry and RT-PCR, separately. Results Compared with the normal control group, stereotyped behavior and exercise behavior were increased, freezing time prolonged, but the content of DA, TH and TH mRNA in the brain tissue were not obviously changed in the model control group. Compared with the model control group, the stereotyped behavior and exercise behavior were decreased, content of DA, TH and TH mRNA in the brain tissue was decreased in the “qiangzhizufang” group. Conclusions The Chinese medicine compound “qiangzhizufang” can improve the behavior in rat models of T