利用SSR标记鉴定棉花杂交种兴杂2号纯度的研究.pdfVIP

Chinese Agricultural Science Bulletin Vo1．24 No．6 2008 June http：／／www．casb．org．ca ·202· 利用SSR标记鉴定棉花杂交种兴杂2号纯度的研究 许兰杰，聂战胜，詹克慧 (河南农业大学农学院，河南郑州450002) 摘 要：杂交棉在削种过程中往往因母本去雄不彻底或漏去雄形成自交铃而导致削种纯度不高，寻找快 速准确的纯度检测方法是杂交棉种子生产和经营急需解决的问题。试验以棉花杂交种兴杂 2号及其亲 本为材料。选用77对异源四倍体棉花SSR引物，采用SSR分子标记技术筛选出双亲间的多态性产物， 并对兴杂2号种子的纯度进行了检测。结果表明，CP55、CP128、CP483、CP478等4对引物在兴杂2号 双亲间扩增出多态性产物。可以准确地鉴别出兴杂 2号及其亲本间的差异，为棉花杂交种兴杂2号的 纯度鉴定提供了一个准确、稳定、快捷、实用的检测方法。 关键词：杂交棉；SSR标记；纯度鉴定；兴杂2号 中图分类号：$562．035．3 文献标识码：A Purity Detection of Cotton Hybrid Xingza No．2 by SSR Markers Xu Lanjie，Nie Zhansheng，Zhan Kehui (Agronomy College ofHenan Agricultural Univers Zhengzhou 450002) Abstract：The purity problem of hybridized cottonseeds resuhed from the no——thorough castration and leaking castration that generates self-boll is usually emerged in the process of seed production，and finding the accu— rate and fast purity testing method is the key to solve it．In this study，seventy—seven pairs of SSR primers from allotetraploid cotton were used to test the purity of cotton hybrid Xingza No．2．The results indicated that four primer pairs such as CP55，CP128，CP483 and CP478 revealed polymorphism between the two parents of Xingza No．2．The four primer pairs could identify accurately the genetic difference between the hybrid and its parents．The accurate，stable and practical method will be very useful to detect purity of hybridized cotton- seeds． Key words：cotton hybrid，SSR markers，purity detection，Xingza No．2 棉花是一个常异花授粉的作物，具有不同于白花 去雄不彻底或漏去雄形成的自交铃[”。如果不能及时 授粉作物和异花授粉作物的遗传特性，所以棉花品种 有效地鉴定出棉花杂交种子的纯度，就会对棉花生产 在生产上有常规种和杂交种之别。随着棉花杂交育种 带来不应有的严重损失。因此，找到一种快捷、高效的 工作的迅速开展，加上棉花杂交种所表现的杂种优势， 棉