凝血混合质控血浆的制备、评价和应用.pdfVIP

880 实用预防医学 2008年6月 第 15卷 第3期 Practical Preventive Medicine，Jun 2008，Vol 15,No．3 文章编号：1006—3110(2008)03—0880—03 凝血混合质控血浆的制备、评价和应用 余启华 ，王孝华 ，易建文 ，佘 鸥 ，欧阳旋 摘要： 目的 探讨凝血混合质控血浆的制备、瓶间差和稳定性及其在室内质量控制(IQC)中的应用。 方法 制备凝血混合质控血浆，一2O℃冰箱保存，选用体检组混合血浆进行瓶间差的测定，同时用Thrombolyzer Compact—X血 凝分析仪上对体检组和患者组混合血浆进行稳定性检测以及常规室内质量控制的开展。 结果 混合血浆的分装引起 的瓶间差很小。体检组混合血浆在一2O℃冰箱保存120 d内PT—s,APTT、Fib稳定性良好，患者组混合血浆的PT—S、 APTT测定值有增加的趋势，Fib降低。患者组混合血浆在 90 d后三个检测项的偏倚(Bias％)虽然可以接受，但变异系数 较大，不再适合作为室内质控物使用。 结论 凝血混合血浆在一定时间内能够满足临床实验室在单值水平上室内质 量控制的使用要求。 关键词： 凝血混合质控血浆；瓶问差；稳定性；室内质量控制(IQC) 中图分类号：R457 文献标识码 ：A Preparation．Evaluation and Application of Mixed Coagulation Control Plasma YU Qi—hua，WANG Xiao—hua，yJ Jian—wen，et a1．(Department of Clinical Laboratory，the Second People’S Hospital of Hunan Province，Changsha 410007， Hunan，China) Abstract： Objective To observe the preparation，the vial—to—vial variability，the stability of the mixed coagulation control plasma，and the application in internal quality control(IQO)． Methods The mixed coagulation control plasma was prepared to store at一20℃ condition．The vial—to—vial variability was determined with the mixed coagulation control plas— ma of volunteers．the stability of the plasma of the patients and the volunteers was determined with thrombolyzer compact—X instrument，Which was applied to internal quality control(IQO)． Results The vial—to—vial variability of the mixed coagu— lation control plasma was little．The values of PT—S，APTT and Fib of the mixed coagulation controI plasma of volunteers were stable within 120 days，while the values of PT—S and APTT of the patients were increased and Fib was decreased：Bi— as％ of the patient group can be accepted when to be stored at一20℃ condition after 90 days，but CV％ was not ideal，and the patient plasma can not be appl