结核抗体的3种检测方法（3 methods for the detection of antibody against tuberculosis）.docVIP

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结核抗体的3种检测方法（3 methods for the detection of antibody against tuberculosis）.doc

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结核抗体的3种检测方法（3 methods for the detection of antibody against tuberculosis）

结核抗体的3种检测方法（3 methods for the detection of antibody against tuberculosis） Clinical evaluation of 3 methods for the determination of anti TB antibody Hu Zhongyi, Jin Anjia, Yin Koufuwang, Hui Feng Keywords tuberculosis; anti tuberculosis antibody; immunochromatographic chromatography; dot immunogold filtration assay; enzyme-linked immunosorbent assay; Use imported immune chromatography anti tuberculosis antibody test card (ICT-TB card) in 128 cases of pulmonary tuberculosis patients serum and 103 control serum were tested, and domestic immunogoldfiltrationassay (DIGFA) and 38 kD antigen ELISA (ELISA) were compared. 1 materials and methods 1.1 serum specimens from 128 patients with active pulmonary tuberculosis from the hospital and Minhang, Fengxian TB inpatients (diagnosis of pulmonary tuberculosis for acid fast bacilli sputum smear examination and sputum, chest X-ray, tuberculin test and clinical manifestations etc.). 53 other respiratory diseases (including lung cancer, bronchitis, asthma, bronchiectasis, etc.) were taken from outpatients. 50 healthy individuals were taken from blood donors. 1.2 cards for Australia reagent ICT-TB ICT diagnostic reagent products, LOT 021857; DIGFA reagent for Shanghai Fuda biological products company products, 980216 batches of recombinant Mycobacterium tuberculosis; preparation of ELISA reagent for Shanghai crystal biological products company 38 kD antigen ELISA test kit, number 984010. 1.3 methods of anti TB antibody test using double blind method, the operation according to the instructions. The examination of sputum tubercle bacillus shall be carried out according to the procedures for operation of the national clinical examination. 2 Result Comparison of 2.13 methods for the determination of anti TB antibody The results of the 3 methods for the determination of 231 serum samples are shown in table 1. The sensitivity, specificity, positive and negative predictive value and coincidence rate of the 3 methods were shown in table 2