两种凝血酶原提取方法的比较（Comparison of the two methods of thrombin extraction）.docVIP

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两种凝血酶原提取方法的比较（Comparison of the two methods of thrombin extraction）.doc

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两种凝血酶原提取方法的比较（Comparison of the two methods of thrombin extraction）

两种凝血酶原提取方法的比较（Comparison of the two methods of thrombin extraction） Comparison of the two methods of thrombin extraction Author: lai yiwu, wu min, Chen man, Chen man, liu Yang [abstract] objective to compare the extraction of thrombin from pig plasma by electrocoagulation and barium citrate adsorption The purity of. Methods: the coagulase was extracted from pig plasma by isoelectric precipitation method and barium acid barium adsorption method Prothrombin is activated by a separate calcium ion to get thrombin, which is determined by the thrombin efficiency and protein concentration To measure the ratio of coagulase to the activity of thrombin. Results the coagulase was extracted by isoelectric precipitation method The coagulase-specific activity obtained after the activity was 11.01 soil 1.54 U/mg, and the coagulation was extracted by the adsorption method of barium acid The activity of thrombin was 130. 17 soil 12.30 U/mg. PC0.01. Conclusion compared with the isoelectric precipitation method, the adsorption method of barium hyalate was extracted from pig plasma The prothrombin has a higher purity. [key words] thrombin; Thrombin; Isoelectric precipitation method; Barium citrate adsorption method [Abstract] Objective To compare the purity of prothrombin Purified by method of precipitating at isoelectricpoint and Method with barium citrate from blood plasma Pig. The Methods Prothrombin was purified by method of precipitating at Isoelectricpoint and method with barium citrate from blood Plasma of pig and activated by Ca2 + only to thrombin. Then the titer And the concentration of protein were measured to get the Specific activity of thrombin.results The specific activity of Thrombin activated by Ca2 + from prothrombin purified by method of The specific method is to be used for isoelectricpoint was 11.01 + 1.54 U/mg The activity of thrombin activated by Ca2 + from prothrombin purified by Method with barium citrate was 130. 17 + 12.30 U/mg Specific activities were analyz