两种凝血酶原提取方法的比较（Comparison of the two methods of thrombin extraction）.doc

下载文档 降价啦

3
0
约1.22万字
约 13页
2017-07-26 发布于河南
举报
版权申诉
保障服务

两种凝血酶原提取方法的比较（Comparison of the two methods of thrombin extraction）.doc

1、本文档共13页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

两种凝血酶原提取方法的比较（Comparison of the two methods of thrombin extraction）

两种凝血酶原提取方法的比较（Comparison of the two methods of thrombin extraction） Comparison of the two methods of thrombin extraction Author: lai yiwu, wu min, Chen man, Chen man, liu Yang [abstract] objective to compare the extraction of thrombin from pig plasma by electrocoagulation and barium citrate adsorption The purity of. Methods: the coagulase was extracted from pig plasma by isoelectric precipitation method and barium acid barium adsorption method Prothrombin is activated by a separate calcium ion to get thrombin, which is determined by the thrombin efficiency and protein concentration To measure the ratio of coagulase to the activity of thrombin. Results the coagulase was extracted by isoelectric precipitation method The coagulase-specific activity obtained after the activity was 11.01 soil 1.54 U/mg, and the coagulation was extracted by the adsorption method of barium acid The activity of thrombin was 130. 17 soil 12.30 U/mg. PC0.01. Conclusion compared with the isoelectric precipitation method, the adsorption method of barium hyalate was extracted from pig plasma The prothrombin has a higher purity. [key words] thrombin; Thrombin; Isoelectric precipitation method; Barium citrate adsorption method [Abstract] Objective To compare the purity of prothrombin Purified by method of precipitating at isoelectricpoint and Method with barium citrate from blood plasma Pig. The Methods Prothrombin was purified by method of precipitating at Isoelectricpoint and method with barium citrate from blood Plasma of pig and activated by Ca2 + only to thrombin. Then the titer And the concentration of protein were measured to get the Specific activity of thrombin.results The specific activity of Thrombin activated by Ca2 + from prothrombin purified by method of The specific method is to be used for isoelectricpoint was 11.01 + 1.54 U/mg The activity of thrombin activated by Ca2 + from prothrombin purified by Method with barium citrate was 130. 17 + 12.30 U/mg Specific activities were analyz